嘉氏羊蹄甲组培技术

Culture technique in vitro of Bauhinia galpinii

为了促进对园林植物嘉氏羊蹄甲的繁殖推广,对其组培技术进行了研究。结果表明,适宜的诱导培养基为MS+6-BA 0.5 mg/L+IBA 0.01 mg/L+GA31.0 mg/L,诱导率达78.2%,腋芽粗壮嫩绿。较好的增殖培养基为MS+6-BA 0.2 mg/L+IBA 0.01 mg/L,增殖系数可达4.63,丛芽生长健壮,叶片舒展翠绿。较好的生根培养基为1/2 MS+IBA 0.4 mg/L、以蛭石代替琼脂粉,20 d生根率可达94.6%,移栽基质为V泥炭土∶V椰糠∶V珍珠岩=3∶1∶1,移栽成活率可达90%以上。

In order to propagate the garden plant Bauhinia galpinii,we developed its culture techniques in vitro. The experiments results indicated that MS ＋ 6-BA 0. 5 mg / L ＋ IBA 0. 01 mg / L ＋ GA31. 0 mg / L was the optimal medium for explants induction of axillary buds,and the induction rate was 78. 2%. The optimal proliferation medium with the robust and orderly buds was MS ＋ 6-BA 0. 2 mg / L ＋ IBA 0. 01 mg / L,the multiplication coefficient of which was 4. 63 roughly. The optimal rooting medium of which agar powder was replaced by vermiculite was 1 /2MS ＋ IBA 0. 4 mg / L,the rooting rate of which was up to 94. 6% after 20 d. The transplanting matrix were peaty soil,coconut coir and perlite（ Vpeaty∶ Vcoconut coir∶ Vperlite=3∶ 1∶ 1）,and the transplanting survival rate was up to 90%.