非小细胞肺癌组织中miR-222、TIMP3的表达变化及意义

Expression changes of miR-222 and TIMP3 in non-small-cell lung cancer and its clinical significance

目的观察非小细胞肺癌组织中miR-222与基质金属蛋白酶抑制剂3(TIMP3)的表达变化,并探讨其意义。方法 88例非小细胞肺癌患者,术中留取肿瘤组织为观察组,取癌旁正常组织48例份为对照组。采用原位杂交和免疫组化实验检测两组miR-222、TIMP3,分析miR-222、TIMP3表达与非小细胞肺癌临床病理参数的关系。结果观察组miR-222阳性表达率为67.05%(59/88),对照组为35.42%(17/48),两组比较,χ2=12.60,P=0.000。观察组TIMP3阳性表达率为40.91%(36/88),对照组为66.67%(32/48),两组比较,χ2=8.24,P=0.004。肺癌组织中miR-222、TIMP3表达呈负相关关系(r=-0.09,P=0.006)。不同临床分期者及不同淋巴结转移情况者肿瘤组织中miR-222、TIMP3表达相比,P均<0.05。结论非小细胞肺癌组织中miR-222阳性表达率增高,TIMP3阳性表达率降低,二者可能与非小细胞肺癌的发生发展有关。

Objective To observe the expression changes of miR-222 and matrix metalloproteinase inhibitor 3 （TIMP3） in the non-small-cell lung cancer tissues and to investigate its clinical significance.Methods Eighty-eight pa-tients with non-small-cell lung cancer were chosen.The cancer tissues （ observation group） and 48 cases of adjacent normal tissues （ control group） were selected.In situ hybridization and immunohistochemistry were used to detect the expression of miR-222 and TIMP3 in the two groups.The correlations among the miR-222 and TIMP3 as well as the clinicopathological parameters of non-small-cell lung cancer patients were analyzed.Results The positive rate of miR-222 was 67.05%（59/88） in the observation group, and 35.42% （17/48） in the control group, and the difference was statistically significant （χ2 =12.60, P=0.000）.The positive rate of TIMP3 was 40.91%（36/88） in the observation group, and 66.67%（32/48） in the control group, and the difference was statistically significant （χ2 =8.24, P=0.004）.In the lung cancer tis-sues, the expression of miR-222 was negatively correlated with the expression of TIMP3 （r=-0.09, P=0.006）.Signifi-cant difference was found in the miR-222 and TIMP3 expression between the cancer tissues with different clinical stages and lymph node metastasis （all P〈0.05）.Conclusion The expression of miR-222 is increased and the expression of TIMP3 is decreased in the non-small-cell lung cancer tissues, and both of them may be related with the occurrence and develop-ment of non-small-cell lung cancer.