VEGF水平与恶性胸腔积液的相关性研究

Study on correlation between VEGF level and malignant pleural effusion

目的探讨血清及胸腔积液中VEGF水平在良、恶性肿瘤中的诊断价值,并评价VEGF-A,C,D在诊断恶性胸腔积液中的临床应用。方法从滨州医学院附属医院住院治疗的患者中,选择肺癌且伴有胸腔积液者,共34例,收集其血清及胸腔积液,采用双抗体夹心ELISA法检测胸腔积液及血清VEGF及VEGF-A,C,D浓度。同时测定34例良性胸腔积液患者的血清及胸腔积液VEGF水平情况,并进一步检测恶性浆膜腔积液患者外周血清,积液上清VEGF-A,C,D的浓度。结果血清及胸腔积液VEGF水平恶性组均显著高于良性组(P<0.05)。另外,初治前肺癌患者血清及胸腔积液VEGF水平,有远处转移组明显高于尚未有远处转移组(P<0.05)。VEGF水平与恶性胸腔积液存在相关性(r=0.878,P<0.05)。VEGF水平与良性胸腔积液无相关性。血清s VEGF-A含量在恶性组及良性组无统计学差别。积液上清p VEGF-A含量在恶性组高于良性积液组(P<0.05),在恶性积液中显示p VEGF-A明显高于s VEGF-A水平(P<0.05)。血清s VEGF-C,s VEGF-D含量在恶性组及良性组无统计学差别。积液上清p VEGF-C,p VEGF-D含量在恶性组及良性组无统计学差别。结论检测血清及胸腔积液中VEGF水平有助于良恶性胸腔积液的诊断和鉴别诊断。积液上清VEGF-A在良恶性积液有差异,提示可能成为良恶性积液的肿瘤标志物。

Objective Methods To explore the value of VEGF levels in serum and pleural effusion for diagnosis of benign and malignant tumors, and evaluate clinical value of VEGF-A, C, D in malignant pleural effusion.Methods Serum and pleural effusion of 34 cases patients with lung cancer were collected in our hospital, the application of ELISA method for the detection of VEGF level in serum and pleural effusion in patients with lung cancer and with benign pleural effusion.VEGF-A, C, D levels were detected.Results VEGF levels in serum and pleural effusion in malignant group were significantly higher than those in benign group（P〈0.05）.In addition, patients with lung cancer before initial treatment, the VEGF levels in serum and pleural effusion of distant metastasis group were significantly higher than those of without distant metastasis group（P〈0.05）.There was a correlation between the level of VEGF and the malignant pleural effusion（r=0.878, P〈0.05）.No correlation existed between VEGF level and benign pleural effusion.The content of sVEGF-A in serum had no statistical difference in cancer group and benign group.Effusion supernatants of pVEGF-A content in lung cancer group were higher than those in benign effusion group（P〈0.05）.pVEGF-A and sVEGF-A levels were similar in benign effusion group. Effusion supernatants pVEGF-A in malignant group was higher than that in benign effusion group（P〈0.05）.pVEGF-A was significantly higher than that of sVEGF-A in malignant effusion（P〈0.05）.Serum VEGF-C, VEGF-D content had no significant difference between cancer group and benign group. pVEGF-C, pVEGF-D content had no significant difference between cancer group and benign group.Conclusion Level of VEGF in serum and pleural effusion detection would help to diagnose and differentially diagnose benign and malignant pleural effusion.Effusion VEGF-A is different in benign and malignant effusion, which may become benign and malignant effusion tumor markers.