八正胶囊的质量标准研究

Study on quality specification of bazheng capsule

目的：建立八正胶囊的质量标准。方法用薄层色谱法鉴别大黄素和大黄酚，并用高效液相色谱法测定样品中大黄素、大黄酚的含量测定。结果薄层色谱斑点清晰，且空白无干扰；大黄素结果表明，大黄素进样量在0．0267～0．4272μg范围内呈良好的线性关系，Y＝1425．7X＋2．1521（r＝0．9998）；大黄酚进样量在0．0417～0．6677μg，Y＝5×106X－8566．2（r＝0．9999）范围内呈现良好的线性关系。加样平均回收率分别为97．35％、98．39％；RSD分别为1．7％、0．4％。结论建立的方法简便，重复性好，可用于八正胶囊的质量标准。

Objective To improve the quality of the standard bazheng capsule.Methods TLC was used for identification of emodin and chrysophanol content determination, and determination of samples by high performance liquid chromatography.Results TLC spots were clear,and the blank without interference.Emodin showed a good linear relationship at range of 0.0267 ~0.4272 μg, Y =1425.7X ＋2.1521 （ r=0.9998 ）;chrysophanol showed a good linear relationship at range of 0.0417 ~0.6677 μg, Y =5 ×106X -8566.2（r=0.9999）.The recovery was 97.35%, 98.39%,and RSD was 1.7%,0.4%.Conclusion The established methods are simple and with good repeatability, and can be used for the quality control of bazheng capluse.