α-氨基酸酯水解酶合成头孢克洛

Enzymatic Synthesis of Cefaclor by α-Amino Acid Ester Hydrolase

用实验室自制的α-氨基酸酯水解酶(AEH)催化合成了头孢克洛。考察了pH值、温度、酶浓度、7-ACCA/D-PGM·HCl投料比、有机溶剂和D-PGM·HCl流加方式等多个因素对该反应的影响。所得最优工艺组合为:pH 6.1,温度15℃,7-ACCA浓度100 mmol/L,投料比1∶1.3,反应体系含25%乙醇,加料方式为侧链流加。优化工艺后头孢克洛的转化率达80%以上,为其酶法合成的进一步发展提供了参考。

The synthesis of cefaclor by self-made α-amino acid ester hydrolase was carried out. The effect of reaction conditions including pH, temperature, enzyme concentration, ratio of 7-ACCA/D-PGM·HC1, organic solvent and feeding of side chain was studied. The optimum conditions were pH 6.1, temperature 15 ℃, concentration of 7-ACCA 100 mmol/L, 7-ACCA/D-PGM·HCI 1 ： 1.3, 25% ethanol-water and continuously adding side chain. Under these conditions, the conversion of cefaclor was over 80%. The study supplied experiment data to the further development of enzymatic synthesis of cefaclor.