L-岩藻糖的生产菌种选育及发酵工艺优化

Strain Screening and Fermentation Production of L-Fucose by Enterobacter sp.

以肠杆菌属(Enterobacter sp.)SIPI-10为出发菌株,经紫外与亚硝基胍(NTG)诱变,筛得1株L-岩藻糖高产突变株SIPI-N79,产量为1 276 mg/L。通过优化发酵培养基组成,得到优化配方(g/L):甘油30.0,胰蛋白胨LP0042 5.0,CaCO3 5.0,L-岩藻糖的摇瓶发酵产量为1 582 mg/L。进一步在5 L发酵罐中进行分批补料培养,当补加150 g葡萄糖时,L-岩藻糖的产量达到4 553 mg/L,较摇瓶发酵水平提高显著。

The starting strain Enterobacter sp. SIPI-10 was mutated with UV and NTG for the production of L-fucose. A high-yield mutant strain SIPI-N79 was obtained and the yield of L-fucose was 1 276 mg/L. By optimizing fermentation medium, SIPI-N79 produced 1 582 mg/L L-fucose in shaking flask contained （g/L） glycerol 30,0, peptone LP0042 5.0, CaCO3 5.0. Furthermore, a fed-batch culture in a 5 L fermentor was conducted. The yield of L-fucose reached to 4 553 mg/L with feeding 150 g of glucose, which showed a significant increase in yield compared with that in shaking flask culture.