去甲斑蝥酸钠脂质微球注射液对荷人肺癌A549移植瘤裸鼠肿瘤生长的抑制作用

Inhibitory effect of sodium norcantharidin lipid microsphere injection on nude mice bearing human carcinoma A549

目的:观察去甲斑蝥酸钠脂质微球注射液对荷人肺癌A549细胞移植瘤裸鼠模型肿瘤生长的抑制作用。方法:在BALB/cnu裸鼠右前肢腋下接种A549细胞株建立人肺癌动物模型,移植11d后采用均衡法随机分为5组,即去甲斑蝥酸钠脂质微球注射液高剂量组(5mg/kg)、中剂量组(2.5mg/kg)、低剂量组(1.25mg/kg)以及阳性对照组(2.5mg/kg去甲斑蝥酸钠注射液)和阴性对照组(等体积空白脂质微球注射液),每组7只裸鼠,给药体积为0.2mL。采用尾静脉注射给药,各组动物于分组后第1、4、8、11、15、18、22天给药,共7次。于给药第7、14、24天检测并计算各组肿瘤体积(Vt)、相对体积(VRT)、增殖率(T/C)等,给药后第24天检测并计算各组肿瘤质量和抑瘤率。结果:去甲斑蝥酸钠脂质微球注射液给药第7天后,各给药组肿瘤体积、相对体积均明显小于阴性对照组(P<0.05),并具有剂量-效应关系(r7d=0.994,r14d=0.993,r24d=0.996,P均<0.05);24d后各剂量组动物肿瘤T/C均小于35%,其中2.5、5mg/kg剂量组和阳性对照组T/C均小于20%;各给药组平均肿瘤质量明显低于阴性对照组(P<0.01),肿瘤生长抑制率均大于50%。结论:去甲斑蝥酸钠脂质微球注射液具有抑制人肺癌A549细胞生长,降低人肺癌A549细胞移植瘤增殖率的作用。

OBJECTIVE： To study the inhibitory effect of sodium norcantharidin lipid microsphere injection on nude mice bearing human carcinoma A549. METHODS： Animal model of human lung cancer was established by inoculating human carcinoma A549 cells to BALB/c nude mice. 11 days after inoculation, mice were randomly divided into control group, positive control group, and other three groups with different doses of sodium norcantharidin lipid microsphere injection. The mice received physiological saline, 2.5 mg/kg sodium norcantharidin injection, and 1.25, 2.5 or 5 mg/kg sodium norcantharidin lipid microsphere injection at 1 d, 4 d, 8 d, 11 d, 15 d, 18 d, and 22 d after grouping. The tumor volume （Vt）, relative volume （VaT）, and the increment rate （T/C） were measured at 7 d, 14 d, and 24 d after the first injection. The weight of tumors and the growth inhibition rate in vivo were measured at 24 d after the first injection. RESULTS ： The tumor volume and the relative volume of all treated groups were much smaller than the group with negative control 7 days after the first injection, and the difference was significant （P〈0.05）. These effects showed drug dose-effect relationships （r7 a=0.994, r14 d=0.993, r24 d=0.996）. The tumor increment rate of all treated groups were lower than 25%, especially the medium dose and the high dose groups which dropped to below 20%. The average tumor weights of the treated groups were lower than the control group （P〈0.01）. The growth inhibition rates were more than 50%. CONCLUSION： Sodium norcantharidin lipid microsphere injection effectively inhibited the growth and reduced increment rate of human carcinoma A549 transplanted in nude mice.