TRPC1基因缺失对小鼠空间学习记忆的影响

The effect of TRPC1 gene deficiency on learning and memory of mice

目的:探讨TRPC1基因对小鼠空间学习和记忆的影响。方法:取4月龄野生S129小鼠(对照)和TRPC1基因敲除的S129小鼠(TRPC1 KO)各10只,采用Morris水迷宫检测其学习和记忆能力。小鼠进行连续5 d的空间探索训练以找到平台位置(定位航行实验),记录每组小鼠每天4个象限的潜伏期。在学习结束后的第7天进行长期记忆的检测(空间探索实验),记录小鼠第1次到达平台位置的时间(探索时间)和游泳轨迹、穿越平台次数、总路程、平均速度、目标象限活动时间百分比和目标象限活动路程百分比等体现小鼠记忆能力的参数,采用t检验比较两组实验结果的差异。结果:在维持5 d的学习阶段,野生对照小鼠和TRPC1 KO小鼠的潜伏期差异无统计学意义(P>0.05)。在长期记忆检测阶段,野生对照小鼠和TRPC1 KO小鼠的潜伏期、穿越平台的次数、平均速度、总路程、目标象限活动时间百分比和目标象限活动路程百分比的差异均无统计学意义(P>0.05)。结论:TRPC1基因敲除未影响4月龄小鼠的学习和记忆能力。

OBJECTIVE： To explore the effect of transient receptor potential C class 1 （TRPC1） gene on spatial learning and memory of mice. METHODS ： Using Morris water maze to perform a consecutive 5-days learning of spatial acquisition （spatial navigation test） on 4-month wild-type （S129） and TRPC1 gene knockout （TRPC1 KO） mice with S129 background （n=10） and record the four quadrants latency independently. Long-term memory on the seventh day after the end of the learning stage was measured, and some parameters that could reflect memory ability of the mice were analyzed as follows： the first time that mice reach the platform （probe time）, swimming trajectories, platform crossing numbers, total distance, average speed, percentage of time in target quadrant and percentage of distance in target quadrant. Independent-sample t test was applied to analyze the behavioral differences between S129 and TRPC1 KO mice. RESULTS： During 5-days learning stage, wild-type mice and TRPC1 KO mice have no significant difference in latency. In the stage of long-term memory measurement, wild-type mice and TRPC1 KO mice displayed no significant difference in probe time, crossing number of platform, average swimming speed, total distance travelled, the percentage of time spent and the percentage of distance travelled in the correct quadrant. CONCLUSION ： TRPCI gene deficiency does not affect spatial learning and memory ability of d-month-old mice.