破壁方法和溶剂对法夫酵母提取物抗氧化活性的影响

Effect of Cell Wall Disrucption Methods and Extraction Solvents on the Antioxidant Activity of Phaffia rhodozyma

选用盐酸、Na OH两种破壁方法以及甲醇、乙醇、丙酮、石油醚/丙酮(体积比3∶7)4种溶剂对法夫酵母进行提取。以铁离子还原能力(FRAP)、1,1-二苯基-2-苦基肼(DPPH)、·OH自由基、ABTS+自由基清除能力来评价提取物的抗氧化活性;同时测定提取物的总类胡萝卜素含量,并对提取物抗氧化活性与总类胡萝卜素含量做相关性分析。结果表明:Na OH破壁提取物的抗氧化能力显著强于盐酸破壁提取物;在4种提取溶剂中,甲醇提取物清除ABTS+自由基、·OH自由基的能力以及铁离子还原能力显著强于其他3种溶剂提取物,而清除DPPH自由基的能力稍弱乙醇提取物,显著强于丙酮、石油醚/丙酮(体积比3∶7)提取物;甲醇提取物中总类胡萝卜质量最大(16.133 mg±0.284 mg),所得浸膏质量最小(0.7107 g),提取物中总类胡萝卜素含量【(22.7±0.4)mg/g DW】显著高于其他3种溶剂提取物;总类胡萝卜素含量与ABTS+清除能力和铁离子还原能力显著相关,相关系数分别是0.9765和0.9616,而与DPPH、·OH自由基清除能力相关性不显著,相关系数分别是0.1185和0.5794。

Hydrochloric acid and sodium hydroxide were used to disrupt the cell wall of. Phaffa rhodozyma. Methanol, ethanol, acetone, petroleum ether/acetone（3：7） were used as extraction solvents. The extracts were screened for their potential antioxidant activity using vitro systems such as free radical-scavenging activity （DPPH, ABTS＋, OH） and ferric reducing/antioxidant power. At the same time, the total carotenoids contents were measured. And correlation analy- sis was done to analyze the relation between total carotenoids contents and antioxidant activity. The results showed that： ①Compared to Phaffia rhodozyma treated with hydrochloric acid, extracts of Phaffia rhodozyma treated with sodium hy- droxide showed obviously higher antioxidant activity. ②Among the selected solvents, the extract treated with methanol showed obviously higher ability of ABTS＋ .OH free radical scavenging and ferric reducing/antioxidant power than the other three extracts. While the ability of scavenging DPPH radical was a bit weaker than ethanol, obviously higher than acetone and petroleum ether/acetone （3：7）. ③The extract treated with methanol had the highest total carotenoids content （22.7±0.4 mg/g DW）. ④Correlations between total carotenoids contents and ABTS＋ scavenging ability, ferric reducing/antioxidant power were significant （the correlative coefficients were 0.9765 and 0.9616, respectively）. While correlations between total carotenoids contents and DPPH, .OH scavenging ability were not significant （the correlative coefficients were 0.1185 and 0.5794, respectively）.