温经通络散外用对奥沙利铂周围神经毒性大鼠神经保护作用的研究

Protective effect of powder for warming channel for removing obstruction in collaterals on nerve in rats with peripheral nerve toxicity induced by oxaliplatin

目的:探讨温经通络散外用对奥沙利铂所致周围神经毒性大鼠神经保护作用。方法:雌性Wistar大鼠随机分为正常组、模型组、中药组,腹腔注射奥沙利铂4mg/kg,建立奥沙利铂周围神经毒性大鼠模型。定期检测温度和机械刺激下大鼠行为学变化,并测定其尾神经感觉神经传导速度(SNCV)、潜伏期,血浆NGF含量以及L5背根神经节神经元的形态分析。结果:模型组出现明显行为学改变(P<0.05),尾神经SNCV、血浆NGF下降(P<0.05),背根神经节神经元核仁固缩明显,偏心核、多核仁核增多(P<0.05)。中药组大鼠行为学明显改善;尾神经SNCV及NGF较模型组均升高(P<0.05)。结论:中药温经通络散外用可改善奥沙利铂所致周围神经毒性大鼠温度和机械刺激下的行为学改变、提高尾神经SNCV以及NGF水平。

Objective： To explore the protective effect of powder for warming channel for removing obstruction in collaterals on nerve of rats with peripheral nerve toxicity induced by oxaliplatin. Methods： Female wistar rats were randomly divided into control group, model group and Chinese medicine group. Peripheral nerve toxicity rat models were established by using intraperitoneal injection with oxaliplatin （4mg/kg）. The temperature, behavior changes of rats under mechanical stimulation, sensory nerve conduction velocity （SNCV） and incubation period were all observed regularly. The contents of nerve growth factors （NGF） and the morphology of neurons of L5 dorsal root ganglion were also observed. Results： In model group： the behavioristics of rats was changed significantly （P〈0.05）; the SNCV of coccygeal nerve and NGF content in plasma were decreased significantly （P〈0.05）; and the pyknotic nucleolus, off-centered cell nucleus and multiple nucleolus nucleus were appeared in dorsal root ganglion （P〈0.05）. In Chinese medicine group： the behavioristics of rats was improved significantly; the SNCV of coccygeal nerve and NGF content in plasma were increased compared with model group （P〈0.05）. Conclusion： External use of powder for warming channel for removing obstruction in collaterals could improve the temperature and changes in behavioristics under mechanical stimulation, and increase the SNCV of coccygeal nerve and NGF content in plasma of rats with peripheral nerve toxicity induced by oxaliplatin.