针刺对心肌缺血再灌注损伤大鼠心肌组织CaMK Ⅱ表达及细胞凋亡的影响

Influence of acupuncture on CaMKⅡ expression and apoptosis of rats with myocardial ischemia reperfusion injury

目的:观察电针手厥阴经内关、郄门穴在缺血再灌注损伤过程中对心肌组织内Ca MKⅡ蛋白含量及心肌细胞凋亡的影响。方法:将Wistar大鼠随机分为假手术组、缺血-再灌注模型Ⅰ组、缺血-再灌注模型Ⅱ组、针刺内关治疗组、针刺郄门治疗组、针刺合谷对照组、Ca MKⅡ抑制剂组(KN-93),除假手术组外,其余各组制备缺血-再灌注模型,并接受电针治疗,摘取心脏;荧光免疫组化法观察Ca MKⅡ蛋白的表达,TUNEL法观察心肌细胞凋亡率。结果:针刺内关治疗组及针刺郄门治疗组Ca MKⅡ平均光密度值高于假手术组而低于缺血-再灌注模型Ⅰ组(P<0.01),心肌细胞凋亡率显著低于两模型组(P<0.01);针刺郄门治疗组和KN-93抑制剂组Ca MKⅡ光密度值和心肌细胞凋亡率与针刺内关治疗组比较无显著差异。结论:针刺在减轻Ca2+超载过程中,心肌细胞凋亡的抑制作用与Ca MKⅡ信号通路密切相关。

Objective： To observe the protein expression of CaMK Ⅱ and myocardial apoptosis in cardiac muscle tissue of rats with myocardial ischemia reperfusion injury by acupuncture in Neiguan （PC6） and Ximen （PC4） points. Methods： Wistar rats were randomly divided into sham-operated group, model Ⅰ group, model Ⅱ group, Neiguan group and Ximen group, Hegu （LI4） control group and KN-93 group. Rats in all the groups except sham-operated group were received ischemia-reperfusion surgery and electro-acupuncture treatment. The expression of CaMK Ⅱ and myocardial apoptosis were observed by using fluorescent immunohistochemical method and TUNEL method respectively. Results： The average optical density of CaMK Ⅱ protein in rats in Neiguan and Ximen group were significantly higher than that of sham-operated group and lower than that of model Ⅰ group （P〈0.01）. The myocardial apoptosis of rats in Neiguan group was significant lower than that of model Ⅰ and Ⅱ group （P〈0.01）. There was no significant differences on the average optical denisity of CaMK Ⅱ and myocardial apoptosis among Ximen group, KN-93 group and Neiguan group. Conclusion： The relations between inhibition of myocardial apoptosis and CaMK Ⅱ signal path are closely in the course of calcium overload remission treated with acupuncture.