京万红软膏对烫伤及创伤大鼠创面愈合、瘢痕形成的影响

Effects of Jingwanhong Ointment on wound healing and cicatrisation in incision and burn wound rats

目的:探讨京万红软膏对烫伤及创伤大鼠创面愈合、瘢痕形成的影响。方法:SD大鼠分为正常组、模型组、京万红基质组、京万红软膏组,每组60只。除正常组外,每组取30只,在脊柱两旁剪去全层皮肤形成两个直径1.5cm的开放圆形创面,另30只100℃水烫伤15s,在脊柱两旁造成两个直径2.3cm的烫伤创面。万红软膏组、京万红基质组分别于创面每日敷药1次,正常组及模型组不用药。造模后第7、14、28天分别取各组大鼠10只,测量创面面积及皮肤组织病理组织形态。结果:与模型组比较,创伤后第7、14天京万红软膏组创面残留面积小于模型组,第28天无显著差异,烫伤后第14天京万红软膏组创面残留面积小于模型组,第7、28天无显著差异。病理组织学显示,与模型组比较,京万红软膏组创伤后大鼠皮肤创面坏死组织少,烫伤后表面坏死组织脱落早;京万红软膏组创伤和烫伤后创面愈合快,愈合过程中炎细胞浸润少,瘢痕较薄,新生上皮形成快,新生皮肤附属器较多。京万红基质组介于京万红软膏组与模型组之间。结论:京万红软膏能促进大鼠皮肤创伤及烫伤后创面愈合,抑制瘢痕形成,促进皮肤附属器新生。

Objective： To investigate the effects of Jingwanhong Ointment on wound healing and cicatrisation in wound and bums rats. Methods： SD rats were divided into four groups： normal group, model group, Jingwanhong matrix group and Jingwanhong Ointment group, with 60 rats in each group. Thirty rats of every group except the normal group were made incisal wound by cutting the full-thickness skin on either side of the vertebral column to form two circular diameter of 1.5cm open wounds. The other 30 rats were heat the skin on both sides of the vertebral column using 100℃ water for 15s to causing two 2.3cm in diameter bum wounds. The wounds of Jingwanhong Ointment group and Jingwanhong matrix group were treated once daily with Jingwanhong Ointment and its matrix independently. The normal group and model group received no medication. The wound contraction, epithelialization, scar size, and histopathology were monitored on day 7, 14 and 28 after incision or burn injury, ten rats each time. Results： The wound area of Jingwanhong Ointment group was smaller than that of the model group at the 7, 14 days after incision. The wound area of the model group, Jingwanhong Ointment group and the Jingwanhong matrix group at the 28 days after incision had no significant difference. We get similar result in the burn injury model. The wound area of Jingwanhong Ointment group was smaller than that of the model group at the 14 days after burn injury. The wound area of the model group, Jingwanhong Ointment group and the Jingwanhong matrix group at the 7, 28 days after incision has no difference. The histopathology result revealed that comparing with the model group, Jingwanhong Ointment group had less necrotic tissue post-traumatic. Jingwanhong Ointment could promote the necrotic tissue separate from the burn wound. The speed of wound healing was faster in Jingwanhong group than in the model group. The inflammatory cell infiltration, granulation tissue formation, and scar formation in Jingwanhong Ointment group was less in Jingwanhong matrix group than those in the model group. The re-epithelialization was faster in Jingwanhong matrix group than in the model group. Jingwanhong matrix group was between Jingwanhong Ointment group and model group. Conclusion： Jingwanhong Ointment could facilitate the wound healing process, inhibit scar formation and promote skin appendages regeneration in wound and burn rats.