巨尾桉叶中原花色素提取工艺比较及不同溶剂萃取物生物活性的研究

Extraction Technology of Proanthocyanidins from Urophylla Leaves and Biological Activities of Different Solvent Extracts

通过正交试验探讨了巨尾桉叶中原花色素的3种提取方法的最佳提取工艺条件,并进行了对比。结果显示,传统溶剂提取法的最优工艺为:60%乙醇、80℃、100 min、料液比1∶14(g∶m L,下同),原花色素得率5.95%;微波辅助提取法的最优工艺为:50%乙醇、微波功率200 W、微波时间4 min、料液比为1:20,得率5.48%;超声波辅助提取法的最优工艺为:60%乙醇、60℃、超声波时间25 min、料液比为1∶14,得率为6.07%。超声波辅助提取法效果最好,时间短,得率高。实验对超声波提取物的不同溶剂萃取物体外抗氧化性和抗肿瘤活性进行了研究,结果表明,乙酸乙酯萃取物抗氧化作用较强,当质量浓度为1.2 g/L时,对DPPH自由基清除率达到96.33%;乙酸乙酯萃取物质量浓度为2 g/L时,对于人肝癌细胞Bel-7404具有很强的抗肿瘤活性,抑制率为56.37%。

The optimum extraction conditions of proanthocyanidins from urophylla leaves using three extraction methods were investigated by using orthogonal experiment. The extracted results were compared. Then, vitro antioxidant and anti-tumor activities of different solvent extracts from ultrasonic extracts were studied. The optimum crafts for traditional solvent extraction method were 60% ethanol,80 ~C, 100 min, ratio of material-liquid 1 ： 14 （g： mL, the same below）, and the obtained yield of proanthocyanidin was 5.95%. The optimum crafts for microwave-assisted extraction were 50% ethanol, microwave power 200 W,4 min for each time,ratio of material-liquid 1 ： 20 with the yield of proanthocyanidin 5.48%. And the optimum crafts for ultrasonic extraction method were 60% ethanol, 60 ~C, ultrasonic time 25 min, ratio of material-liquid 1 ： 14, and the yield of proanthocyanidin was 6.07%. Ultrasonic assisted extraction worked best, due to the short extract time and the high yield. The results of antioxidant test showed that the ethyl acetate extracts from ultrasonic extracts exhibited the highest antioxidant activity, as well as the maximum of DPPH radical scavenging ratio was 96.33% with the concentration of 1.2 g/L among different solvent extracts. Experimental results of antitumor activity of different solvent extracts showed that the ethyl acetate extract possessed highest antitumor activity for human hepatoma cell Bel-74（M with the concentration of 2 g/L,and the inhibition rate was 56.37%.