食管癌组织中Skp2和p27的表达及临床意义

Expression and clinical significance of Skp2 and p27 in the tissues of esophageal carcinoma

目的探讨S期激酶相关蛋白2(Skp2)和p27在食管癌组织中的表达及其临床意义。方法免疫组化法检测82例食管癌和10例癌旁组织中Skp2、p27的表达;分析Skp2、p27蛋白表达率与临床病理特征的关系;采用Spearman秩相关法分析两者的关系。结果 Skp2在食管癌和癌旁组织中的阳性表达率分别为67.07%(55/82)和20.00%(2/10),差异有统计学意义(P<0.01)。p27在食管癌和癌旁组织中的阳性表达率分别35.37%(29/82)和90.00%(9/10),差异具有统计学意义(P<0.01)。Skp2蛋白表达与性别、年龄、肿瘤部位均无关(P>0.05),与分化程度、肿瘤侵犯深度、淋巴结转移和TNM分期有关(P<0.05)。p27蛋白表达与性别、年龄、肿瘤部位、肿瘤侵犯深度、TNM分期无关,与分化程度、淋巴结转移有关(P<0.05)。Skp2、p27蛋白表达呈负相关(r=-0.750,P<0.001)。结论 Skp2和p27参与正常食管组织向食管癌的演变,可能与食管癌的发生和发展有关。

Objective To investigate the expression and clinical significance of S-phase kinase-associated protein 2 （Skp2） and p27 in the esophageal carcinoma. Methods Immunohistochemistry method was used to detect expression of Skp2 and p27 in 82 cases of esophageal carcinoma and 10 cases of norrnal esophageal epithelial tissue adjacent to esophageal cancer. The correlation be- tween expressions of Skp2 and p27 protein with clinicopathological features was analyzed by chi-square test. Correlation analysis was made by Spearman correlation analysis. Results Positive rates of Skp2 and p27 were 67.07%（55/82） and 35.37% （29/82） in e- sophageal carcinoma, significant different from 20. 00% （2/10） and 90. 00% （9/10） in normal tissues （P〈0. 01 ）. Skp2 expression was not associated with gender, age and tumor site, but with differentiation grades, the depth of tumor invasion, lymph node metastasis and clinical TNM stages（P〈0. 05） ; p27 expression was not associated with gender, age, tumor site, the depth of tumor invasion and clini- cal TNM stages, but with differentiation grades and lymph node metastasis（P〈0.05）.There was a negative correlation between Skp2 and p27 expression（ r=-0. 750,P〈0. 001 ）. Conclusion Skp2 and p27 expression involve in the development and progression from normal esophageal tissue to esophageal carcinoma, and may be correlated to the onset and progress of esophageal carcinoma.