摘要
应用虎红平板凝集试验(Rose-Bengal Plate Agglutination Test,RBPT)、衣原体、弓形虫和绵羊肺炎支原体间接血凝试验(Indirect Hemagglutination Assay,IHA),对采自青海省河南县部分乡的200份欧拉型藏羊血清样品进行了布鲁氏菌、衣原体、弓形虫和绵羊肺炎支原体的血清学抗体检测。结果:在所有被检血清样品中检测出弓形虫抗体阳性血清数12份,阳性率为6.0%;绵羊肺炎支原体血清抗体阳性数为1份,阳性率为0.5%,未检出布鲁氏菌和衣原体抗体阳性血清。此外,对于抗体阳性的血清采用PCR方法进行了抗原检测,结果表明青海省河南县地区欧拉型藏羊中存在弓形虫和绵羊肺炎支原体感染。
To investigate the existence prevalence of Brucellosis, Chlamydiosis, Toxoplasmosis and Mycoplasma Ovipneumoniae of 200 Oum-type of Tibetan sheep serum samples in Henan County of Qinghai Province, Rose-Bengal Plate Agglutination Test （RBPT）, Cldamydia, Toxoplasma, and Mycoplasma Ovipneumoniae Indirect Hemagglutination Assay （IHA） were used to detect antibody of these disease. The zesults showed that 12 of the 200 samples were Toxoplasmosis positive, 1 samples were Mycoplasma Ovipneumoniae positive and the positive rates were 6 percent and 0.5 percent, respectively. Brucellosis and Chlamycllos/s were not detected. In addition, the antigen were detected by PCR method using the sample of serum antibody positive. In conclusion, the Oura-type of Tibetan sheep in Henan County were infected by Toxoplasma and Mycoplasma Ovipneumoniae.
出处
《青海畜牧兽医杂志》
2015年第4期8-10,共3页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
现代农业产业技术体系建设专项经费资助(CARS-04-08B)
青海省现代农牧业产业科技创新平台-羊产业科技创新平台
