摘要
传染性造血器官坏死病(IHNV)是一种危害极其严重的鱼类疾病。本实验用一步RT-PCR法和套式PCR法对IHNV的基因进行扩增,通过扩增片段的琼脂糖电泳分析,来判定病鱼是否携带IHNV。实验结果表明,通过一步法RT-PCR可扩增出IHNV的693bp片段;通过套式PCR法可扩增出IHNV的786bp片段和323bp片段。两种方法均能够检测待测样品中含有IHNV。比较发现一步RT-PCR法比套式PCR法更加快速、简便、敏感,减少了被污染机会。
Infectious hematopoietic necrosis virus（IHNV） is a very severe kind of fish diseases .This ex‐periment use one-step RT -PCR and nested PCR to amplify gene of IHNV,through amplified gene fragments＆#39;agarose electrophoresis analysis ,we determine whether the diseased fish carry IHNV .The experimental results show that by one-step RT -PCR we can amplify the IH‐NV 693 bp fragments;Through nested PCR we can amplify the IHNV segments of 786 bp and 323 bp fragment .Two test all can test the samples which have IHNV .Compared one -step RT -PCR with nested PCR ,it show s that one-step RT -PCR method is more rapid ,simpler ,more sensitive and it can reduce pollution .
出处
《河北渔业》
2015年第8期48-51,71,共5页
