高效液相色谱法测定愈伤灵胶囊中黄曲霉毒素G_2,G_1,B_2,B_1含量

Determination of Aflatoxin G_2,G_1,B_2,and B_1 in Yushangling Capsules by HPLC

目的建立测定愈伤灵胶囊中黄曲霉毒素G2,G1,B2,B1含量的高效液相色谱（HPLC）柱后光化学衍生法。方法样品经70%甲醇提取,免疫亲和柱净化,HPLC柱后光化学衍生,荧光检测器定量,并用超高效液相色谱-串联质谱（UPLC-MS/MS）法对阳性样品进行确认。结果黄曲霉毒素G2,G1,B2,B1含量线性范围分别是3.54～35.40 pg,7.08～70.80 pg,2.10～21.00 pg,6.24～62.40 pg（r〉0.999）,回收率均在70%～120%。结论该法简便、灵敏、结果准确,适用于愈伤灵胶囊中黄曲霉毒素的检测。

Objective To establish a method for determination of aflatoxin G2, G1, B2, B1 in Yushangling capsules by HPLC. Methods The samples were extracted with 70% methanol and purified with immunoaffinity column, then analyzed by HPLC with fluorescence de-tection. The positive samples were identified by ultra performance liquid chromatography - tandem mass spectrometry. Results Aflatoxin G2, G1, B2, B1 showed a good linear relationship at a range of 3. 54 - 35. 40 pg, 7. 08 - 70. 80 pg, 2. 10 - 21. 00 pg, 6. 24 - 62. 40 pg （ r〉 0. 999） . The recoveries were between 70% - 120% . Conclusion The method is simple, sensitive and accurate, which is suitable for determination of four aflatoxins in Yushangling Capsules.