脓毒症大鼠急性肺损伤血清抵抗素及IL-6的变化及其意义

The change of serum resistin,IL-6 on the acute lung injury of sepsis rats and its significance

目的研究脓毒症大鼠急性肺损伤(ALI)血清抵抗素及白介素-6(IL-6)的水平,以探讨抵抗素及IL-6与ALI的关系及可能机制。方法 Wistar雄性大鼠45只随机分为正常对照组、假手术组和模型组各15只。静脉注射脂多糖(10mg/kg)复制ALI的动物模型,分别观察6、12、24h后活杀。测定肺湿/干重比,用化学发光法检测血清IL-6及ELISA法检测抵抗素水平。结果与对照组及假手术组相比,模型组肺湿/干重比,肺病理组织评分、血清IL-6水平、抵抗素水平均明显增高(P<0.05)。肺组织损伤程度与血清IL-6和抵抗素水平呈正相关(r值分别为0.739、0.874,P<0.05)。结论抵抗素可能参与了脓毒症所致的肺损伤过程。

Objective To study the serum level of resistin and IL‐6 in the sepsis‐induced acute lung injury （ALI） in rats and to probe the relation of IL‐6 and resistin to ALI and its possible mechanism .Methods The forty‐five Wistar rats were randomly as‐signed to three groups ,a control group ,a sham group and a model group .ALI was induced by intravenous injective of LPS （10 mg/kg） and those animals were killed after 6 hours ,12 hours ,24 hours .The lung wet/dry weight ratio ,serum IL‐6 was detected by chemiluminescence and resistin levels were detected by ELISA .Results Compared with the control group and the sham group ,the lung wet/dry weight ratio ,lung pathologic tissue score ,the serum level of resistin and IL‐6 were increased（P〈0 .05） .Positive cor‐relation was observed between resistin and IL‐6 in different time .Conclusion Resistin possibly participate the process of sepsis in‐duces ALI .