加味四妙散对兔膝骨关节炎IL-6和bFGF的影响

Effects on IL-6 and BFGF of the Rabbits' Knee Arthritises by Modified Simiao Power

目的:观察加味四妙散对兔膝骨关节炎白细胞介素-6(interleukin-6,IL-6)、碱性成纤维细胞生长因子(basic fibroblast grow th factor,b FGF)的影响,并分析b FGF、IL-6与Mankin’s评分是否有相关性。方法:选取健康清洁大白兔36只,随机选取12只为对照组,共12个关节,行假手术。其余24只应用改良Hultu法建兔膝骨关节炎模型,共24个关节;造模成功后,随机分为模型组、受试组各12只,受试组给予加味四妙散汤剂,空白组及模型组给予等量生理盐水喂服,10周后,取右后肢膝关节内胫骨平台的软骨作为标本。1观察标本软骨的大体形态,关节内关节积液情况,并给出大体评分;2通过光镜观察标本软骨并评分;3通过光镜观察HE染色后标本软骨的细胞数量、组织结构和潮线的完整情况,参照Mankin’s标准进行评分;4免疫组织化学法测定b FGF、IL-6在标本软骨内的表达情况,并将b FGF、IL-6的表达水平与Mankin’s评分进行Pearson相关性分析。结果:1受试组的大体评分和Mankin’s评分与模型组比较,差异无统计学意义(P>0.05);2造模组的大体评分和Mankin’s评分均高于对照组,差异有统计学意义(P<0.05),其中以模型组最为显著;3各造模组全层软骨中IL-6表达较对照组显著升高,以模型组为最(P<0.05);受试组IL-6表达低于模型组(P<0.05);4模型组b FGF表达高于对照组(P<0.05),受试组b FGF表达高于模型组(P<0.05);5 IL-6表达与Mankin’s评分指数呈弱正相关,r=0.183(P<0.05);b FGF表达与Mankin’s评分指数呈弱负相关,r=-0.283(P<0.05)。结论:加味四妙散能调节软骨中IL-6和b FGF表达;软骨病理改变的Mankin’s评分与IL-6表达呈正相关,与b FGF表达呈负相关。

Objective ：To study the effects on IL（ interleukin-6, IL-6）and BFGF of the rabbits＇ knee arthritis by modified Simiao power, and analyzing the grades of BFGF, IL-6 and Mankin＇s, whether they have the correlation. Methods ：36 clean white rabbits were randomly difided into blank group（12） ,which has 12 arthrosises do sham-operation. The rest of the 24 rabbits to be built the rabbits model of knee arthritis by improved Hultu method, a total of 24 joints. After the molding, they were divided into model group （12）and experimen- ted group（12） ,the experimented group was given modified Simiao San,the blank group and modles were given normal saline. 10 weeks later, After 10 weeks,in the right leg knee joint cartilage as a specimen of tibial plateau. ①To observe the general form of specimen cartilage and intra-articular joint effusion, the general score is given;② observing the specimen cartilage by light microscope and give a score;③observing the HE staining of specimens after cartilage cells, organization structure and the tide line by light microscope, and give a score by reference Mankin＇s rating standards ;④bFGF, IL-6 in the expression of specimens in cartilage by immune histochemical method determination ,and the expression of bFGF and IL-6 level and Mankin＇s score for Pearson correlation analysis. Results：①The general score and Mankin＇s score In the experimented group compared with model group,there was no statistically significant difference（P 〉 0. 05 ） ;②in the modle group, gross score and Mankin＇s score were higher than the control group, the difference was statistically significant（P 〈 0. 05）, of which the most model group significantly; ③ the building modules of full-thickness cartilage IL-6 exPression significantly increased than the control group, model group as the most （ P 〈 0.05 ） ; IL-6 express group is lower than the model group （ P 〈 0.05 ） ; ④model group bFGF expression is higher than the control group（ P 〈 0.05 ）, a group bFGF expression is higher than the model group （P 〈 0. 05 ） ;⑤ expression and Mankin IL-6＇s weak positive correlated to the score index （r = 0. 183, P 〈 0.05 ） ;BFGF ex- pression and Mankin＇s weak negative correlated to the score index（r = -0. 283 ,P 〈 0. 05 ）. Conclusion：Modified Simiao San can ad- just cartilage of IL-6 and bFGF expression. Cartilage pathological changes of Mankin＇s score and IL-6 expression were positively correla- ted, and negatively correlated with bFGF expression.