摘要
目的:建立同时测定槐花中芦丁、槲皮素、染料木素含量的方法。方法:运用反相高效液相色谱法。色谱柱为Hypersil gold C18(4.6 mm×250 mm,5μm),甲醇-(体积分数)0.2%冰醋酸溶液为流动相梯度洗脱,检测波长370 nm。结果:芦丁、槲皮素、染料木素在2.04~40.08 mg·L-1、2.42~48.4 mg·L-1、0.8~16.0 mg·L-1范围内呈良好线性关系;三种成分平均回收率分别为101.9%(RSD=3.49%,n=6)、103.6%(RSD=1.76%,n=6)、101.9%(RSD=2.85%,n=6)。结论:本方法简便,准确性、稳定性好,适用于槐米的分析测定。
Objective:To establish the methods of Rutin, Quercetin, Genistein of Sophorae Flos. Methods:HPLC method was used, the de- termination was performed on Hypersil gold C18 (250 mm × 4.6 mm,5 μm) with mobile phase consisted of methanol -0.2% acetic acid solution at detection wavelength of 370 nm. Results:Rutin, Quercetin, Genistein showed good linear relationship. The average recov- ery was 101.9% ( RSD = 3.49 %, n = 6), 103.6% ( RSD = 1.76% , n = 6) , 101.9% ( RSD = 2.85 % , n = 6) respectively. Conclusion : The method is simple, accurate and good stability, which can be used in assay determination of sophora flower bud.
出处
《中医学报》
CAS
2015年第8期1176-1178,共3页
Acta Chinese Medicine
关键词
槐花
芦丁
槲皮素
染料木素
高效液相色谱法
flos sophorae
rutin
quercetin
genistein
high performance liquid chromatography