产番茄红素红酵母筛选及其培养条件的优化

Screening of Rhodotorula strain for high yield of lycopene and optimization of its cultivation

以实现番茄红素的微生物发酵生产为目标,从土壤中分离筛选出一株产番茄红素的菌株,初步鉴定为红酵母。以该红酵母为出发菌株,对其进行紫外诱变,选育得到优良高产番茄红素突变株Zk1-1。并对培养条件进行优化,试验结果表明,在发酵周期120h、葡萄糖40g/L、酵母膏10g/L、Mg SO4 0.03g/L的条件下,番茄红素产量达20.25mg/L,比未优化前的产量(11.00mg/L)提高了84%,效果显著。

In order to use microbial fermentation method to produce lycopene, one microbial strain was selected by screening. The strain was preliminary identified as rhodotorula and used as a starter strain in ultraviolet mutagenesis selection. The mutant strains Zk1-1 with high yield in lycopene was obtained. The optimum fermentation conditions were determined. The results showed that the fermentation time 120h, glucose 40g/L, yeast extract 10g/L, and MgSO4 0.03g/L. The yield of lycopene was 20.25mg/L, 84% more compared with starter （11.00mg/L） .