1025例涉案手机和电脑接头类检材的DNA检验分析

DNA Analysis of 1025 Touch Samples from Various Appliance Connectors

本文采用改良硅珠法,对自2012年以来实验室受理的1025例涉案手机、电脑接头类检材(手机充电器接头156例,电脑网线水晶接头416例、电脑电源线接头155例、电脑显示器数据线接头22例、鼠标、键盘等USB接口类检材276例)进行分析,以检出性别及13个以上STR基因座同时图谱峰值≥200 RFU的检材计算,1025例检材检出率为87.0%,其中手机充电器接头检出率为93.6%、电脑网线水晶接头检出率为84.4%、电脑电源线接头检出率为82.0%、电脑显示器数据线接头检出率为95.5%、键盘等USB接口类检材检出率为89.5%。本研究证实,该改良硅珠法便捷、省时高效、检验效果理想,适用于法医微量物证DNA检验。

Touch DNA is now often encountered in forensic investigation. Its successful acquisition is often crucial for solving the relevant cases. In this paper, 1025 pieces of touch DNA samples tested in our laboratory since 2012 are reviewed with the purpose to find some regularity. These samples were collected from various electric connectors consisting of mobile phones’ （156 pieces）, net cables’ （416 pieces）, power cables’ （155 pieces）, displays’ （22 pieces）, and USBs’ （276 pieces）. The DNA was extracted with modified method of silica, amplified with AmpFLSTR？ Identifiler？ Plus PCR Kit, electrophoresed with ABI3500XL. Data were analyzed with GeneMapper ID-X. The results showed that the detection rate was 93.6% for mobile phones’ connectors, 84.4% for net cables’, 82.0% for power cables’, 95.5% for displays’, and 89.5% for USBs’, with a total detection rate of 87.0% for all the 1,025 pieces of touch DNA samples. The method applied here is suitable to tackle the samples of touch DNA.