粘虫转录组中SSR位点的信息分析

The Information Analysis of SSR Loci in the Mythimna separate(Walker)Transcriptome

粘虫 Mythimna separata（Walker）是一种迁飞性害虫，严重危害玉米、水稻、小麦等粮食作物。 SSR 是指以1～6个核苷酸为基本重复单位的串联重复 DNA 序列。 SSR 位点的信息分析为粘虫扩散、迁飞和交配等行为分子机制的研究以及粘虫的综合防治奠定理论基础。本研究基于高通量测序获得的粘虫转录组数据，利用软件 msatcom‐mander 发掘粘虫 SSR 位点。结果从20776条转录组 Unigenes 中共搜索出400个 SSR ，分布于372条 Unigenes 中。在粘虫转录组 SSR 中，三核苷酸重复的数量最为丰富，有271个；其次是二核苷酸和单核苷酸重复，分别是70个和49个；四至六核苷酸重复的数量都很少，共10个。粘虫转录组 SSR 共包含24种重复基元，其中 CCG／CGG 是优势重复基元类型，有69个；其次是 AAG／CTT ，有57个。 CG／CG 有18个，在二核苷酸重复基元中所占的比例达到25.7％。此研究发掘到的 SSR 位点将为粘虫遗传图谱的构建、遗传多样性分析、亲缘关系分析等提供丰富的分子标记。

Mythimna separata（Walker） is a kind of migratory pests and causes serious damage to the crops such as corn ,wheat ,rice and so on.Simple sequence repeat （SSR） is tandemly repeated motif of 1 ~ 6 nucleotide. The informa‐tion analysis of SSR loci in the Mythimnaseparate （Walker） establishes a theoretical basis for the research of molecular mechanisms such as its diffusion ,journey and mating behavior ,as well as its integrated control.Based on the construc‐ted transcriptome database in Mythimna separata ,the SSR loci were explored by the software msatcommander. In to‐tal ,400 SSR loci were explored from 20 776 transcriptome unigenes ,and they were distributed in 372 unigenes. Among these SSR loci ,trinucleotide were the most abundant repeats ,of which were 271 ,followed by dinucleotide and mononu‐cleotide repeats ,of which were 70 and 49 ,respectively. Tetra‐ 、penta‐and hexanucleotide were 10 in all. There were 24 kinds of repeated motif types in Mythimna separata transcriptome SSR.CCG/CGG was the most advantages repeat motif types （69） ,then AAG/CTT （57）. There were 18 CG/CG repeated motif types which accounted for 25.3% of the dinucleotide repeat motif types. The SSR loci which were discovered in this study would benefit a lot for the construc‐tion of gene mapping ,the research of genetic diversity and the parentage analysis.