黄姑鱼基因组大小和染色体物理长度的测定

Genome size and physical length of chromosomes in Nibea albiflora

该研究以鸡(Gallus gallus)红细胞含量为标准,采用流式细胞术首次测定了黄姑鱼(Nibea albiflora)基因组大小;利用荧光染色和显微图像分析软件测定了24对染色体的相对面积和累积光密度(IOD),并基于IOD估算了各对染色体的物理长度。结果显示,黄姑鱼基因组大小为(595.70±24.08)Mb,DNA质量约为(0.61±0.02)pg。黄姑鱼基因组包括24对端部着丝粒染色体,估测物理长度的最小值为(16.85±4.13)Mb,最大值为(31.95±4.23)Mb。染色体的估测物理长度与相对面积、相对长度均成正线性相关。研究结果为分析石首鱼科基因组大小的进化分歧规律以及即将开展的黄姑鱼全基因组测序工作提供了必要的基础数据,也为黄姑鱼染色体识别与配对提供了新的参考依据。

Taking chicken （ Callus gallus） red blood cells as internal reference standard, we measured the genome size of Nibea albi- flora by using flow cytometry. The relative area and integrated optical density （IOD） of 24 pairs of chromosomes were determined using fluorescence staining and microscopic image analysis software, and the physical length of each pair of chromosomes was estimated based on the IOD values. The genome size of N. albiflora was estimated as （595.70 ± 24. 08 ） Mb, and the DNA content was （0. 61 ± 0. 02） pg. The genome of N. albiflora comprised 24 pairs of telocentric chromosomes, with the physical length ranging from （ 16. 85 ±4. 13） Mb to （31.95 ± 4. 23 ） Mb. The physical lengths of chromosomes were correlated linearly with the relative area and relative lengths. The results provide basic data for analysis of evolution rule of genome size in Sciaenidae and the forthcoming whole genome se- quencing of N. albiflora, as well as for identifying and paring of chromosomes.