转多基因库安托杨非靶目标材性性状分析

Variation of Non-target Wood Properties of Multi-gene Transgenic Populus × euramericana ‘Guariento'

随着基因工程技术的发展，转基因技术在林木品种改良上得到了广泛的应用和迅速发展，已经开展了30多个树种的转基因研究，改良性状主要集中在抗除草剂、抗虫、降低木质素合成、增加纤维素合成、重金属积累以及加速生物量积累等方面。近年来研究发现将外源基因转入植物后，常引起非靶目标变异，表现出与外源基因表达似乎无关的表型和农艺性状的变异，同时还伴随遗传基础的改变。

In this study, the transgenic poplar （Populus × euramericana‘Guariento＇） harboring five exogenous genes （SacB, Vgb, BtCry3A, OC-I and JERF36#） and the control plants were selected, comparing the wood property variation of 5-year-old trees, and trying to understand whether the transgenic of exogenous genes produce unintended effects. The results showed that the transgenic of exogenous genes caused a wide range of variation in non-target wood properties, the variations were found not only between transgenic and wild-type lines, but also among transgenic lines. Compared with the control, the transgenic lines, the contents of holocellulose and cellulose increased, the total lignin and acid soluble lignin decreased, the fiber length, the fiber width, the microfibril angle and the acid insoluble lignin varied uncertainly. It is concluded that the introduced exogenous gene could generate unintended effects on corre- sponding non-target target traits.