心血瘀阻证动态演变过程大鼠模型的建立及方证验证评价

Establishment of Rat Model of Heart Blood Stasis Syndrome Dynamic Evolution Process and Evaluation of the Model with Prescriptions and Syndromes Verification

目的复制心血瘀阻证大鼠模型动态形成过程并进行方证验证。方法采用高脂饲养与冠状动脉左前降支结扎术结合的方法建立3阶段模型,即第一阶段"血瘀证前期"模型,第二阶段"亚血瘀证期"模型,第三阶段"心血瘀阻证期"模型,并设相应模型对照组及假手术组、阳性药物干预对照组、阴性药物干预对照组,每组8只。成模后采用阳性药物、阴性药物干预法验证动物模型,阳性药物干预组和阴性药物干预组大鼠分别在心血瘀阻证期造模后给予养心通脉片溶液3.25 g/(kg·d)和四逆散溶液3.00 g/(kg·d)灌胃,心血瘀阻证期组给予纯净水(1 ml/100 g)灌胃,共7天。观察各组大鼠中医表征积分,检测血液流变学、血脂指标。结果血瘀证前期组、亚血瘀证期组、心血瘀阻证期组中医表征积分均较本模型相应的对照组升高(P<0.05);心血瘀阻证期组、亚血瘀证期组血脂各指标均较本模型相应的对照组升高(P<0.05);血瘀证前期组、亚血瘀证期组与各自模型对照组比较血浆黏度水平升高(P<0.05)。血浆黏度和全血黏度中切比较,阳性药物干预组低于阴性药物干预组和心血瘀阻证期组(P<0.05)。结论通过3阶段造模法以及方证验证,初步建立了心血瘀阻证动态演变的实验动物模型。

Objective Replicate dynamic evolution process of rat models with heart blood ＇stasis syndrome and make prescriptions and syndromes verification. Methods Models of 3 stages bined method of high fat feeding and ligation of left anterior descending branch of were established adopting the comcoronary. Pre-blood stasis syndrome model was at the first stage. Sub-blood stasis syndrome model was at the second stage. Heart blood stasis syndrome model was at the third stage. Model control group, shamoperation group, positive drug intervention control group and negative drug intervention control group were also established, with 8 rats in each group. After modeling successfully, rat models were verified by applying positive and negative drug intervention method. Rats of positive drug intervention control group and negative drug intervention control group were given solution of Yangxin Tongmai Tables （养心通脉片） 3.25g/ （kg · d） and Sini San （四逆散） 3.00g/ （kg · d）, respectively through gavage after modeling at the stage of heart blood stasis syndrome. Rats of heart blood stasis syndrome stage group were given purified water （ 1 ml/ 100 g） through gavage for 7 days. Rats＇ characterization scores of Chinese Medicine in each group were observed. Hemorheological indexes and blood lipid levels were detected. Results The characterization scores of Chinese medicine in pre-blood stasis syndrome stage group, sub-blood stasis syndrome stage group and heart blood stasis syndrome stage group were higher than those in corresponding control groups （P 〈 0. 05 ）. Blood lipid levels in heart blood stasis syndrome stage group and sub-blood stasis syndrome stage group were higher than those in corresponding control groups （P 〈 0. 05 ）. Serum viscosity level in pre-blood stasis syndrome stage group and sub-blood stasis syndrome stage group was higher than that in corresponding control groups （ P 〈 0. 05）. Compared with plasma viscosity and medium of whole blood viscosity, positive drug intervention control group was lower than negative drug interven- tion control group and heart blood stasis syndrome stage group （P 〈 0. 05 ）. Conclusion models of heart blood stasis syndrome dynamic evolution process were preliminarily established Experimental animal by 3 stages modeling method and prescriptions and syndromes verification.