当归芍药散对药物性肝损伤小鼠的保护作用及其机制研究

Protective Effect of Danggui Shaoyao San on Drug-Induced Liver Injury in Mice and Its Mechanism

观察当归芍药散对异烟肼(INH)和利福平(RFP)合用致小鼠药物性肝损伤的保护作用及其机制。将小鼠随机分为正常组、模型组、当归芍药散高、中、低剂量组(32,16,8mg/kg)和联苯双酯组(200mg/kg)。采用INH和RFP合用复制小鼠药物性肝损伤模型,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST),同时测定肝匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)含量;HE染色法观察肝组织病理学变化,分析当归芍药散的保肝作用及其可能机制。结果表明当归芍药散可降低INH和RFP联用所致药物性肝损伤小鼠肝脏系数,显著降低小鼠血清中ALT(P<0.01),降低AST(P<0.05)含量;肝脏MDA含量明显降低(P<0.05),SOD活力显著提高(P<0.05),GSH-Px含量呈增高的趋势;病理学检查表明当归芍药散能显著减轻肝损伤程度。文章指出当归芍药散对药物性肝损伤小鼠具有一定的保护作用,其机制可能与其抗氧化作用有关。

To investigate the protective effect and mechanismof saponins of Danggui Shaoyao San（DSS）on drug-induced liver injury in mice. Method mice were randomly divided into six groups,control group,low,middle,high-dose（8,16,32mg/kg）of DSS,model group and bifendate group（200mg/kg）.The model of drug-induced liver injury wasinduced by intragastric administrationof isoniazid（INH） and rifampin（RFP） in mice.The levels of alanineaminotransferase（ALT）,aspartate transaminase（AST）in serum and the content of superoxide dismutase（SOD）,malondialdehyde（MDA）,glutathione peroxidase（GSH-Px）inliver homogenate were assayed by spectrophotometry.The pathological changesof hepatic tissueswere observed by hematoxylin and eosin（HE）staining.Result The liverindex DSS middle,high-dose groups was lower than the model group（P0.05 or P0.01）.Compared with the modelgroup,the levels of ALT and AST In DSS middle,high-dose groups were significantly decreased（ P0.05 or P0.01）.The content of MDA DSS middle,high-dose groups was also lower than that in the model group（P0.05 or P0.01）,while the activity of SOD and GSH-Px in DSS middle,high-dose groups was higher than the model group（P0.05 orP0.01）.Hepatic tissues stained with HE showed that PNS could remark ablyre lieve liver injury.The article points outDSS has protective effect on drug-induced liver injury in mice,which may be related toantioxidant effect.