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右美托咪定对内毒素血症大鼠急性肾损伤的影响 被引量:1

Effect of dexmedetomidine on acute kidney injury in endotoxemic rats
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摘要 目的 探讨右美托咪定对内毒素血症大鼠急性肾损伤的影响.方法 健康雄性清洁级SD大鼠30只,4~6月龄,体重180 ~ 220 g,采用随机数字表法分为3组(n=10):对照组(C组)、脂多糖(LPS)组(L组)和右美托咪定组(D组).L组静脉注射LPS 5 mg/kg制备内毒素血症模型.D组静脉注射LPS 5 mg/kg后,静脉注射右美托咪定负荷量7μg/kg,15 min后以5μg· kg-1·h-1的速率静脉输注6 h,L组和C组给予等容量生理盐水.LPS给药结束后6h时取股静脉血样,检测血清Cr、BUN、TNF-α和IL-6的浓度.LPS给药结束后24 h时处死动物,取肾组织,观察病理学结果,采用Western blot法测定紧密连接蛋白ZO-1和occludin的表达水平.结果 与C组比较,L组和D组血清Cr、BUN、TNF-α和IL-6的浓度升高,肾组织ZO-1和occludin的表达下调(P<0.05);与L组比较,D组血清Cr、BUN、TNF-α和IL-6的浓度降低,肾组织ZO-1和occludin的表达上调(P<0.05).D组肾组织病理学损伤较L组减轻.结论 右美托咪定可减轻内毒素血症大鼠急性肾损伤. Objective To investigate the effect of dexmedetomidine on acute kidney injury in endotoxemic rats.Methods Thirty adult male Sprague-Dawley rats,aged 4-6 months,weighing 180-220 g,were randomly divided into 3 groups (n =10 each) using a random number table:control group (group C),lipopolysaccharide group (group L),and dexmedetomidine (group D).Lipopolysaccharide (LPS) 5 mg/kg was injected slowly into the femoral vein to establish the model of endotoxemic in rats anesthetized with chloral hydrate.In group D,after LPS injection,a loading dose of dexmedetomidine 7 μg/kg was injected intravenously,and 15 min later dexmedetomidine was infused for 6 h at 5 μg · kg-1 · h-1,while the equal volume of normal saline was given in L and C groups.At 6 h after the end of LPS administration,blood samples were collected from the femoral vein for determination of serum creatinine (Cr),blood urea nitrogen (BUN),tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) concentrations.At 24 h after the end of LPS administration,the animals were sacrificed and kidneys were removed for microscopic examination and for determination of the expression of tight junction proteins ZO-1 and occludin in renal tissues by Western blot.Results Compared with group C,the serum Cr,BUN,TNF-α and IL-6 concentrations were significantly increased,and the expression of ZO-1 and occluding was down-regulated in L and D groups.Compared with group L,the serum Cr,BUN,TNF-α and IL-6 concentrations were significantly decreased,the expression of ZO-1 and occluding was up-regulated,and the pathological changes of kidneys were mitigated in D group.Conclusion Dexmedetomidine can alleviate acute kidney injury in endotoxemic rats.
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2015年第4期496-498,共3页 Chinese Journal of Anesthesiology
基金 辽宁省科技攻关项目(2013225220)
关键词 右美托咪啶 内毒素血症 Dexmedetomidine Endotoxemia Kidney
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