不同孔径的羟基磷灰石对组织工程骨血管化促进效果的研究

The effect of different pore sized hydroxyapatite for promoting bone vascularization in tissue engineering

目的探讨不同孔径的羟基磷灰石材料对组织工程骨血管化效果的影响。方法采用Wistar雄性大鼠,随机分为A、B、C 3组,分别在其背部皮下植入孔径为200-300、350-450、500-600μm的羟基磷灰石生物陶瓷（复合4μg骨形态发生蛋白）,大小约5mm×5mm×1mm,重约40mg。分别于植入后第1、2、3、4周后处死动物,取出植入物及周围组织,采用苏木素-伊红染色组织学观察,分析局部新生血管化情况。结果 3组不同时间点的血管化面积,组内比较：A组第2、3周差异有统计学意义、B、C组不同时间点差异有统计学意义（P〈0.05）;组间比较：术后1周,仅C组有血管化面积;术后2-4周,B、C组增加的面积高于A组（P〈0.05）,C组可见大量新生血管并形成清晰可见的骨小梁。结论孔径为500-600μm的羟基磷灰石生物陶瓷能够更好地促进组织工程骨的血管化。

Objective To investigate the effect of different pore sized hydroxyapatite for promoting bone vascularization in tissue engineering.Methods Male Wistar rats were randomly divided into three groups,named group A,B and C,which were implanted hydroxyapatite bioceramics compositing 4μg bone morphogenetic protein with different aperture of 200-300,350-450,500-600μm in the back subcutaneously.The size of each block was 5mm×5mm×1mm in a weight about of 40.0mg.After implantation,the animals were killed and the implants and the surrounding tissue were taken out at the first,second,third and forth week respectively.HE staining of histological analysis was used to detect the situation of local neovascularization.Results There was significant difference between second and third week in group A.Comparing the area of vascularization at different time points in group B and group C,there were significant difference in the comparison of intragroup（P〈0.05）.During the first week after surgery,there was only group C that had the area of vascularization.During the second and forth week after operation,the area of vascularization in group B and group C were significant higher than group A（P〈0.05）.The C group showed a great deal of newborn blood vessels and clear formation of bone trabeculae.Conclusion The hydroxyapatite bioceramics of 500-600μm could better promote vascalarization of tissue engineering in bone.