浸染法改良细菌芽胞染色实验的探索

Exploration on a modified dip-staining method for bacterial spores staining

传统芽胞染色法常用孔雀绿染液长时间加热使芽胞着色,该方法用在教学上学生不易操作,成功率低,且孔雀绿染液有毒,加热时容易四溅,对环境造成污染。因此,研究采用一种新的染色法——浸染法,对比孔雀绿浓度及浸染时间对染色效果的影响。结果表明,以10%的孔雀绿为初染剂,浸染10min时染色效果最佳。采用浸染法操作简单易行,染色效果对比鲜明,学生更易于区分菌体与芽胞的结构,且安全环保。

Continued-heating malachite green dye is usually used in traditional spore staining method to color the spore.This method is difficult to operate and has a low successful rate.Besides,the malachite green dye is poisonous,when being heated it could splash then cause pollutions.This study uses the dip-staining method to improve the traditional spore staining method,and compares the staining effects between different malachite green concentrations and different staining time.The results show that the best staining results could be achieved with 10% malachite green dye,staining 10 minutes.This improved method is easy to operate,the staining effect is obvious,and the structure of mycelium and spore is easier to be distinguished and much more safe and green.