助仙丹对多囊卵巢模型大鼠卵巢功能及形态的影响

Influence of Zhuxian Dan on the ovarian function and morphology of the polycystic ovary syndrome model rats

目的观察助仙丹对多囊卵巢大鼠模型卵巢形态和性激素的影响。方法将60只大鼠随机分为2组,空白对照8只,另52只大鼠采用避孕硅胶棒联合皮下注射人绒毛膜促性腺激素方法进行造模,连续监测两个动情周期,选出造模成功大鼠30只,随机分为三组:助仙丹组(10只)、炔雌醇环丙孕酮片组(10只)、模型组(10只),称重,记为用药前大鼠体质量,助仙丹组、炔雌醇环丙孕酮片组分别给予助仙丹配方颗粒和炔雌醇环丙孕酮片灌胃,模型组及空白对照组给予等量自来水灌胃,14天后再次称重,记为用药后体质量,后腹主动脉取血,检测血清雄激素浓度,并摘除双侧卵巢。利用单因素方差分析的统计学方法,观察各组大鼠用药前后体质量变化,卵巢质量变化,及血清雄激素浓度变化,并观察闭锁卵泡数量、颗粒细胞层数目,卵泡发育状态等卵巢形态变化。结果与模型组比较,炔雌醇环丙孕酮片组大鼠治疗前后体质量增加值明显降低,有显著差异(P<0.05),炔雌醇环丙孕酮片组大鼠卵巢质量与模型组比较,有显著差异(P<0.05);各组大鼠雄激素水平未见明显差异。助仙丹颗粒配方组大鼠卵巢切片与模型组大鼠相比:闭锁卵泡数量减少,卵巢颗粒细胞层数明显增加,可见正常发育阶段卵泡及成熟黄体组织。结论助仙丹可改善多囊卵巢综合征大鼠卵巢形态、促进正常排卵。

Objective To investigate the effect of traditional Chinese medicine modified Zhuxian Dan（TCM-ZXD） on ovary tissues pathology and improving sex hormone in rat models of polycystic ovary syndrome（PCOS）. Methods 60 rats were randomly divided into 2 groups, 52 rats in pathological model group and 8 in normal control group. Pathological model group was subcutaneous implanted of progesterone gel rod combined with HCG for building PCOS models. According to the vaginal smear, 30 successful model rats were selected and then randomly divided into three subgroups： TCM-ZXD group （10 rats）, Diane-35 group （10 rats）, model group （10 rats）. All the rats were weighed, recorded as body weight before treatment. TCM-ZXD group and Diane-35 group were given the right amount of formula granules of TCM-ZXD and Diane-35, while the model group and the control group were given the same amount of water. After 14 days of therapy, the rats were weighed again, recorded as body weight after treatment.Blood was collected from abdominal aorta, then T in serum was determined. The weight of the ovary was recorded and ovarian morphology was observed. The results were analyzed by one-way anova. Result Compared with the model group, the added value of body weight of Diane-35 group before and after the treatment decreased significantly （ P〈0. 05 ）, there was a significant change in the Diane-35 group of the rats ovarian weight （ P〈0.05 ）. There was no obvious difference in androgen levels in rats between these groups. According to the Ovary paraffin, TCM-ZXD group could increase the layers of ovarian granulosa cells and the number of luteal tissue, reduce the number of locked follicles. Conclusion Zhuxian Dan can improve the ovarian morphology and promote ovulation in rats with PCOS.