毛细支气管炎患儿血清单核细胞趋化因子水平和外周血淋巴细胞表面趋化因子受体3的表达

Detection of Mig in serum and CXCR3 on lymphocytes of peripheral blood of infants with bronchiolitis

目的观察毛细支气管炎患儿血清单核细胞趋化因子(Mig)的水平及外周血淋巴细胞表面趋化因子受体3(CXCR3)的表达。方法选取住院毛细支气管炎患儿55例,按有无特异性体质分为特应性组和非特应性组,以同年龄门诊健康体检儿童26例为对照组。采用流式细胞术测定外周血CD4+、CD8+T淋巴细胞表面CXCR3(CD183)的表达,ELISA法测定血清中CXCR3配体Mig的水平。结果特应性组、非特应性组及对照组的外周血CD4+T细胞表面CD183+的表达分别为(16.39±4.13)%、(14.39±3.74)%和(11.17±3.13)%,差异有统计学意义(P<0.05);外周血CD8+T细胞表面CD183+的表达分别为(67.18±10.57)%、(61.44±11.46)%和(51.19±5.42)%,差异有统计学意义(P<0.05);血清Mig水平分别为(99.67±35.77)ng/L、(120.28±32.28)ng/L和(63.90±15.82)ng/L,差异有统计学意义(P<0.05)。结论 Mig和CXCR3均参与了毛细支气管炎的发病过程;CXCR3可能与特应性体质相关。

Objective To explore the expression of monokine induced by interferon- 7 （Mig） in serum and chemokine receptor 3（CXCR3）on lymphocytes of peripheral blood in children with bronchiolitis. Methods In this study, 55 patients with bronchiolitis in our hospital were randomly recuited, and were divided into two groups： atopic group and non-atopic group. Of the same age 26 healthy children had been enrolled randomly as control group. The level of CXCR3 expression （CD183） on lymphocytes of peripheral blood was detected by flow cytometry in all children. The level of Mig in serum was assayed by ELISA. Results The level of CD183 expression on the CD3＋CD4＋T lymphocytes in atopic group and non-atopic group（16.39±4.13%,14.39±3.74 %）were higher than that of control group （11.17±3.13%, P〈0.05）, CD183＋CD4＋/CD4＋% in atopic group were higher than that in non-atopic group（P〈0.05）. The level of CD 183 expression on CD3＋CD8＋T lymphocytes in atopic group and non-atopic group（67.18±10.57 %, 61.44±11.46 %）were higher than that of control group （51.19±5.42 %, P〈0.05）, CD183＋CD8＋/CD8＋% in atopic group were significantly higher than that in non-atopic group（P〈0.05）. The level of Mig in serum of children with bronchiolitis in atopic group and non-atopic group（99.67±35.77ng/L, 120.28±32.28ng/L）were significantly higher than that in control group （ 63.90± 15.82 ng/L, P〈 0.05 ）. The level of Mig in non-atopic group was higher than that in atopic group, there was significant difference（P〈0.05）. Conclusions Mig and CXCR3 are involved in the pathogenesis of bronchiolitis, and CXCR3 may relate to allergic factors.