响应面法优化碱溶性龙胆多糖的提取研究

Optimization Extraction of Alkali-solubility Polysaccharide from Gentiana scabra Bunge by Response Surface Methodology

为深入开发提取碱溶性多糖,采用响应面法(RSM)即碱提醇沉法对龙胆多糖进行提取,研究提取次数、提取温度、提取时间、液料比、碱浓度等因素对多糖提取率的影响。结果表明:在单因素试验基础上,结合响应面分析确定了提取碱溶性龙胆多糖的最适工艺条件为,提取温度74℃,料液比1∶25,提取时间1.8h,提取次数为1次,碱浓度为9%。模型预测的碱溶性龙胆多糖的提取率为4.4%,在此最佳提取参数下,碱溶性龙胆多糖的试验提取率为(4.32±0.124)%,与预期值相符。

For the further development of the alkali-soluble polysaccharide extraction,response surface methodology （RSM） was used to optimize the extraction conditions of alkaline solubility polysaccbarides from the Gentiana scabra Bunge. The method of alkali extraction and alcohol sink was used to extract polysaccharides. The effect of extraction times, extraction temperature, extraction time, ratio of water to raw material, and NaOH concentration on the yield of Gentiana scabra Bunge polysaccharides were investigated. The results showed that combining single factor test and response surface methodology,the optimum extraction conditions were chosen as follows., extraction temperature was 74℃, ratio of water to raw material was 1：25, extraction time was 1.8 h,one time extraction and the NaOH concentration was 9 %. Under the optimize conditions, the GSP predicted value of yield was 4.4 %,and the GSP experimental yield was 4.32±0. 124%, the results were reasonable with expectation.