黑木耳发酵产纤维素酶的研究

Study on Cellulase Fermentation of Auricularia auricular

黑木耳作为食用菌中的木腐型菌类,其降解纤维素类物质分泌纤维素酶系的能力很强,对黑木耳发酵产纤维素酶的条件进行优化。结果表明:经优化黑木耳产纤维素酶系中CMC酶的最佳发酵条件为,在添加补充成分蔗糖20g·L-1的发酵液中,接入1cm2斜面PDA菌种,25℃,先静置2d,再于120r·min-1条件下摇床发酵6d产酶最高。

Auricularia auricular is a kind of wood rot fungi,it can degrade the cellulose,and the ability to secrete cellulase is very strong, the condition of black fungus fermentation producing CMC enzyme was optimized. The results showed that optimum fermentation conditions was as follow： 20 g·L^-1 sucrose into the liquid fermention medium,access 1 cm^2 PDA strains,25℃ ,first static femention for 2 d,then 120 r·min^-1 for 6 d.