不同类型人乳头瘤病毒多重感染与宫颈病变的相关性研究

Relationship between different types of HPV multiple infection and cervical lesions

目的探讨不同类型人乳头瘤病毒(HPV)多重感染与宫颈病变的相关性。方法应用基因芯片技术对200例宫颈病变患者石蜡组织标本进行24种HPV基因型进行检测,以组织病理学诊断作为宫颈病变确诊的金标准为依据。结果 200例标本中,HPV总感染率为51.50%(103/200),其中慢性宫颈炎、鳞状上皮内低度病变(CIN1)、鳞状上皮内高度病变(CIN3)和宫颈鳞状细胞癌的HPV感染率分别为22/57(38.60%)、12/33(36.36%)、44/81(54.32%)、25/29(86.21%),与宫颈病变密切相关(P<0.01);不同宫颈病变中单一HPV感染率明显高于多重HPV感染率(P<0.05);高危型单一感染组HPV感染率与宫颈病变不相关(P>0.05),多重感染、高危型感染、高危型多重感染、低危型多重感染、高低混合型多重感染的各组HPV感染率在不同级别宫颈病变差异有统计学意义(P<0.05)。结论 HPV感染率与宫颈病变的不同程度密切相关,持续高危型感染HPV和多重感染HPV是加重宫颈病变的重要因素。

Objective To explore the relationship between different types of human papilomavirus（HPV） mul‐tiple infection and cervical lesions .Methods The gene chip technology was applied to detect 24 types of HPV geno‐types in the paraffin tissue specimens from 200 patients with cervical lesions ,the histopathological diagnosis was used as the gold standard of cervical lesion diagnosis .Results In 200 specimens ,the total infection rate of HPV was 51 .50% （103/200） ,in which the HPV infection rates in chronic cervictis ,low‐CIN1 ,high‐CIN3 and squamous cell carcinoma were 22/57（38 .60% ） ,12/33（36 .36% ） ,44/81（54 .32% ） and 25/29（86 .21% ） ,respectively ,and were cor‐related with the cervical lesions（P〈 0 .01） ;In different cervical lesions ,single HPV infection was significantly higher than multiple HPV infection（P 〈0 .05） ;The HPV infection rate in the high‐risk type single HPV infection group had no correlated with the cervical lesions（P 〉 0 .05） .The HPV infection rates in the multiple infection ,high‐risk HPV infection ,high‐risk HPV multiple infection ,low‐risk HPV multiple infection ,and high and low risk mixed HPV infection groups had statistically significant differences among different grades of cervical lesions（P〈0 .05） .Conclu‐sion The infection rate of HPV is correlated with the different degrees of cervical lesion .The persistent high‐risk HPV infection and multiple HPV infection are the important factors for aggravating the cervical lesions .