阪崎克罗诺杆菌的16S rDNA鉴定分型

Identification and Typing of Cronobacter Sakazakii by 16S rDNA Sequencing

阪崎克罗诺杆菌(Cronobacter sakazakii)是一种重要的食源性条件致病菌,易引发新生儿、早产儿、低体重新生儿和免疫力低下的婴幼儿产生严重的脑膜炎、菌血症和坏死性小肠结肠炎。本研究以分离自不同来源、通过生化鉴定的37株阪崎克罗诺杆菌作为研究对象,利用16S rDNA基因测序的方法对阪崎克罗诺杆菌进行鉴定和分型。结果表明:ES4并非为阪崎克罗诺杆菌,说明采用16S rDNA基因测序进行阪崎克罗诺杆菌的鉴定更为可靠;通过构建系统发育树分析,分离菌株位于同一系统发育分支;根据序列同源性比对,可将这一分支下的所有菌株分成7个子群,这表明16S rDNA基因测序可以对阪崎克罗诺杆菌进行分型,进一步揭示其系统发育关系。

Cronobacter sakazakii is an important food-borne conditional pathogenic bacteria, which can easily cause newborn, prema- ture, low birth weight infant and low immunity babies severe necrotizing enterocolitis, bacteremia and meningitis. Totally 37 C. sakazakiiiso- lated from different sources and passed by biochemical identification were chosen as the research objects, C. sakazakii were identified and classified using 16S rDNA gene sequencing method. Results showed that the ES4 was not part of C. sakazakii , based on 16S rDNA gene se- quencing to identify the C. sakazakii more reliable. By constructing phylogenetic tree analysis, the results showed that isolates were in the same branch of the phylogenetic tree, and the isolates could be divided into 7 subgroup in this branch after comparison sequence homology, which showed that C. sakazakii could be parted by 16 S rDNA gene sequencing, further revealed the relationship between system develop- ment.