促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞的保护作用

The protective effect of erythropoietin on cultured retinal Müller cells injured by high glucose

目的探讨促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞的保护作用。方法体外传代培养大鼠视网膜Müller细胞,分组为N组(正常对照),G组(25 mmol/L葡萄糖),E1G组(1×104 IU/L rh EPO+25 mmol/L葡萄糖),E2G组(2×104 IU/L rh EPO+25 mmol/L葡萄糖),E4G组(4×104 IU/L rh EPO+25 mmol/L葡萄糖),MTT比色法比较五组视网膜Müller细胞活力的改变。酶联免疫吸附试验检测各组细胞caspase-3蛋白表达的情况。结果 G组、E1G组、E2G组、E4G组细胞活力均低于N组,E1G组、E2G组、E4G组细胞活力高于G组,E4G组高于E2G组,E2G组高于E1G组。酶联免疫吸附试验检测结果显示G组caspase-3表达高于N组,rh EPO干预各组caspase-3表达均低于G组,并随EPO干预浓度增高表达降低,但均高于N组。结论促红细胞生成素对高浓度葡萄糖培养大鼠视网膜Müller细胞有保护作用。

Objective To investigate the protective effect of erythropoietin on cultured retinal Muller cells injured by high glucose. Methods Neonatal rats＇ retinal Miiller cells of serial subculfivafion were divided into N （normal control group）, G （25 mmol/L glucose） group, E1G （l×l0^4 IU/L recombinant human erythropoietin＋25 mmol/L glucose） group, E2G （2×10^4 IU/L recombinant human erythropoietin＋25 mmol/L glucose） group and E4G （4×l0^4IU/L recombinant human erythropoietin＋25 mmol/L glucose） group. MTT assay was applied to detect cells viability in five groups. The expression of caspase-3 was detected with enzyme linked immunosorbent assay. Results Cell viability of G group, EIG group, E2G group and EaC group were lower than that of N group. Cell viability of E1G group, E2G group and E4C group were higher than that of G group, E4G group was higher than that of E2G group and E2G group was higher than that of EIG group. The expression of caspase-3 in E1G group, E2G group and E4G group were lower than that in G group which was high compared with N group. The expression of caspase-3 in E1G group, E2G group and E4G group were lower than that in G group which was high compared with N group. With the increase of rhEPO, the expression of caspase-3 was reducing. Conclusion Erythropoietin pretreatment could effectively protect the cultured retinal Miiller cells from injuries induced by high glucose.