MMP-9与TIMP-1在复发性流产中的基因表达及相关性研究

Study of gene expression in recurrent spontaneous abortion and the relationship between MMP-9 and TIMP-1

目的检测MMP-9、TIMP-1在复发性流产绒毛组织中的mRNA相对表达量,研究MMP-9和TIMP-1的表达失衡与复发性流产的相关性。方法选取2013年4~12月我院妇科门诊正常早孕和复发性流产妇女的绒毛组织各19例和28例,RT-PCR检测MMP-9、TIMP-1的相对表达量。结果复发性流产组绒毛MMP-9mRNA的相对表达量为4.11±0.42,正常早孕组绒毛MMP-9mRNA的相对表达量为2.45±0.36,两组比较差异有统计学意义（P〈0.01）;复发性流产组绒毛TIMP-1mRNA相对表达量为7.62±1.54,正常早孕组绒毛TIMP-1相对表达量为8.41±1.02,两组比较,差异无统计学意义（P〉0.05）。结论复发性流产绒毛组织中的MMP-9mRNA表达量显著下降,TIMP-1mRNA表达量升高,MMP-9和TIMP-1的表达失衡参与了复发性流产的发生、发展。

Objective To detect the relative expression of MMP-9,TIMP-1 in recurrent spontaneous abortion villus tis-sues of mRNA,to study the correlation of MMP-9 and TIMP-1 expression imbalance and recurrent spontaneous abor-tion. Methods Normal early pregnancy （19 cases）and recurrent spontaneous abortion （28 cases） villus tissues in our hospital from April to December 2013 were selected.The relative expression of RT-PCR was detected in MMP-9, TIMP-1. Results The recurrent spontaneous abortion group of relative expression of MMP-9 mRNA was 4.11＋0.42, normal pregnancy group of the relative expression of MMP-9 mRNA was 2.45＋0.36,to be compared with the normal group,the expression was significantly decreased,there was significant difference（P〈0.01）.Recurrent spontaneous abortion group of the relative expression of TIMP-1 mRNA was 7.62＋1.54,normal group of the relative expression of TIMP-1 was 8.41＋1.02,to be compared with the normal group,the expression was increased,but the difference was not significant （P〉0.05）. Conclusion In recurrent spontaneous abortion villus tissues,the expression of MMP-9 mRNA significantly decreases,the expression of TIMP-1 mRNA increases,the unbalanced expression of MMP-9 and TIMP-1 takes part in the occurrence and development of recurrent spontaneous abortion.