断眼天牛DNA条码试剂盒的研制

DNA barcoding identification kit technology for Tetropium species

【目的】为了加强对检疫口岸截获木材中的断眼天牛种类进行准确、快速的鉴定,克服单纯依赖于形态学方法为主的局限性,我们研发了断眼天牛属DNA条码试剂盒检测技术。【方法】本研究基于线粒体COⅠ基因(线粒体细胞色素氧化酶亚基Ⅰ),采用加拿大条码中心开发的昆虫及植物DNA提取方法,对基因序列分析,获得碱基位点规律。【结果】断眼天牛属中的9个常截获种类取得了标记性碱基位点。【结论】检疫中常截获的断眼天牛属中的9个种通过标记性碱基位点得到区分;研发了断眼天牛属DNA条码试剂盒检测技术,为进出境口岸的检疫工作提供一种新的鉴定手段。

[Objectives] To rapidly and accurately identify Tetropium spp., which are invasive pests commonly intercepted at quarantine ports in shipments of wood, and improve on current, mainly morphologically-based, methods of identifying these pests. [Methods] A DNA barcode detection kit was developed for Tetropium spp. based on the mitochondrial CO I gene. DNA extraction methods followed those of the Canadian Plant Development DNA barcode Center. [Results] Nine base sites were obtained from Tetropium spp. [Conclusion] Analysis of different genetic characteristics among Tetropium spp. allowed nine species to be identified. The newly developed DNA barcoding identification kit provides a new way of identifying this pest at oorts of entrv and exit.