利用小麦苗饲养稻纵卷叶螟的方法

A method for rearing the rice leaf folder(Cnaphalocrocis medinalis) using wheat seedlings

【目的】目前，虽然已有利用玉米苗饲养稻纵卷叶螟Cnaphalocrocis medinalis的成功方法，但仍然存在着玉米苗冬天不易种植、饲养成本较高的问题，因此，本文研究了一种在室内以小麦苗替代玉米苗连续继代饲养稻纵卷叶螟的方法，从而使室内饲养该虫变得更简便和经济。【方法】将稻纵卷叶螟初孵幼虫接至小麦苗上饲养，采用生命表方法测定稻纵卷叶螟第1代及第4～6代的生物学特征，同时将小麦苗和玉米苗饲养的稻纵卷叶螟分别回接至水稻苗上，分别建立其在水稻苗上的生命表。【结果】稻纵卷叶螟可以在室内用小麦苗进行连续继代饲养。稻纵卷叶螟在小麦苗上连续饲养6代后，除蛹重轻于玉米苗上饲养的外，其它生物学特征，如存活率、历期、性比、产卵量、卵孵化率等都与玉米苗上饲养的无显著差异。与玉米苗上饲养的稻纵卷叶螟相同，小麦苗上饲养了6代的稻纵卷叶螟仍能很好地在水稻上生长发育、存活和繁殖。【结论】利用小麦苗饲养的稻纵卷叶螟的生物学特征与玉米苗饲养的相似，可以利用小麦苗进行稻纵卷叶螟的室内保种与大量饲养。

[Objectivesl To develop a relatively convenient and cheap method of culturing the rice leaf folder （RLF） Cnaphalocrocis medinalis using wheat seedlings. Although a successful method for rearing the RLF on maize seedlings has been established this has some obvious limitations, such as the difficulty of planting maize seedlings in winter and the high price of maize seeds. [Methods] Life tables of the RLF on wheat and maize seedlings were compiled and key parameters compared. RLF larvae reared on wheat and maize seedlings were transferred onto rice seedlings to examine their relative fitness using the life table method. [Results] The RLF can complete its life cycle on wheat seedlings, and the survival and lifespan of larvae and pupae, sex ratio, lifetime fecundity and hatching rate of insects raised on wheat seedlings and maize were similar, except that the pupal weight of insects raised on wheat seedlings was significantly lighter. RLFs that had been reared on wheat for six generations could live normally on rice seedlings. [Conclusion] The biological traits of RLFs reared on wheat or maize seedlings were similar. Wheat seedlings can be used to culture large numbers of RLFs under laboratory conditions.