批量细胞吸附固定装置的研制

Batch cell adsorbed immobilization device

针对目前胞浆内单精子注射(ICSI)效率低、对操作人员技术要求较高的问题,研制了一种批量细胞吸附固定装置。首先,基于微流道低雷诺数的特点,通过COMSOL软件进行流体仿真,分析了细胞吸附固定的机理;然后,搭建了压力控制系统,以实时检测并调节微管道内的压力,利用所设计制作的细胞吸附固定装置,结合显微操作系统对小鼠卵细胞进行大量实验并分析影响吸附固定效果的因素;最后,进行单细胞受力分析,推导得出压力泵流量与管道内压力之间、压力与细胞变形量之间的关系。得到的实验数据证明了利用细胞吸附固定装置可以实现批量细胞的自动固定,提高了注射效率。

According to the low efficiency and high skill requirement for human operator of Intraeytoplasmic Sperm Injection （ICSI）, a batch cell absorbed immobilization device was developed. Firstly, based on the little Reynolds number of micro-tunnels, the principle of cell absorbed immobilization was analyzed via COMSOL liquid simulation. Subsequently, the pressure control module was built to measure and adjust the pressure on the micro-tunnel in real time, the device was applied in the micromanipu- lation system to conduct a series of experiments with mouse embryos, which resulted for analyzing the affecting factors of cell immobilization. Finally, force analysis of single cell was performed to deduce the relationship between the pump flow and the pressure on micro-tunnel as well as the relationship between pressure and cell deformation. The experiment data demonstrated that automated batch cell absorbed immobilization can be implemented with this device consequently to increase the efficiency of ICSI.