高温胁迫对家蚕血液蛋白质组的影响

Proteome analysis of silkworm haemolymph under heat stress

高温饲育环境是造成夏秋蚕减产的主要原因之一。为了研究持续高温对家蚕血液蛋白质组的变化,采用双向电泳和质谱技术,分析了高温胁迫后家蚕原种和杂交种血液蛋白的表达差异。结果发现,有87.79%的蛋白点表现出加性模式,有6.73%表现为超显性,而5.49%则表现为显性不足。在鉴定的28个差异蛋白中,热激结合蛋白(h2)、氨基酰化酶(h9)、血液保幼激素结合蛋白(h14)、羟基丙酮酸异构酶(h23)和家蚕生物钟蛋白(h24)5个蛋白在杂交种和原种中差异较大。这些结果暗示了杂交种可能是通过热激关联蛋白的高度表达来直接抵御高温胁迫,通过加速能量代谢和生长发育来避免高温带来的伤害。

High temperature is the main reason responsible for the reduction of silkworm production in summer or au- tumn. In order to observe the proteome change of silkworm haemolymph under long time heat treatment, two-dimen- sional eleetrophoresis and mass spectrometry technologies were employed in the present study. The results showed that a total of 87.79%, 6. 73% and 5.49% protein spots showed additivity, over-dominance and under-dominance patterns, respectively. Twenty-eight differentially expressed protein spots were identified by peptide mass fingerprint- ing. Among these, 5 spots including heat shock cognate protein （ h2 ）, aminoacylase （ h9 ）, hemolymph juvenile hormone binding protein （h14）, hydroxypyruvate isomerase （h23） and time interval measuring enzyme-esterase A4 （ h24）, were differentially expressed in hybrid and its parents. All of these illustrated that hybrid might directly pro- duce high expression of heat shock cognate protein to resist high temperature stress, and accelerate the step of energy metabolism and growth and development to avoid high temperature damage.