我国人工栽培和野生黑色羊肚菌的菌种鉴定及系统发育分析

Strain identification and phylogenetic analysis of cultivated and wild strains of Morchella belonging to Elata Clade in China

采用常规形态学原理,结合核糖体DNA转录间隔区(ITS)序列分析技术,对我国4个羊肚菌主要栽培菌株和5个主要采集自川渝地区的野生分离物进行菌株鉴定和系统发育分析,研究遵循羊肚菌MLST数据库的序列鉴定程序,基于系统研究过的可靠序列信息,对获得的ITS序列进行逐一比对,选取相似度靠前的信息,构建系统发育树.结果表明,目前我国大面积种植的羊肚菌分别为梯纹羊肚菌、六妹羊肚菌和七妹羊肚菌;5个野生种质分别鉴定为梯纹羊肚菌和高羊肚菌.

Applying regular morphology combined with the technology of ITS sequence analysis,the identification and phylogenetic analysis of the 4 cultivated strains and 5 wild morel isolates mainly collected from Sichuan province and Chongqing municipality were carried out. The study followed the sequence identification program required by data bank of multilocus sequence typing（ MLST）. The phylogenetic tree was constructed with reliable sequences obtained from BLAST one-by-one comparison. The results suggested that the cultivated morel varieties were identified as Morchella importuna,M. sextelata and M. septimelata. In addition,5 wild isolates were identified as M. elata and M. importuna,respectively.