摘要
研究一株分离自红树林真菌PH1008胞外多糖(exopolysaccharide,EPS)对小鼠免疫功能的影响,评价其在免疫功能方面的应用价值。体外实验采用四甲基偶氮唑盐(MTT)法检测该菌胞外多糖对小鼠脾细胞增殖的刺激作用和观察其对小鼠NK细胞杀伤活性的影响;体内实验以三组不同剂量EPS连续小鼠灌胃28 d后,进行脏体指数测定。分离自海南红树林的真菌PH1008胞外多糖在体外能刺激小鼠脾细胞增殖,具有促进NK细胞杀伤K526细胞的能力;增加小鼠脏体指数,增强固有免疫功能。产自海南红树林的真菌PH1008初步鉴定为近平滑假丝酵母菌,其胞外多糖具有增强小鼠免疫功能。
Research on EPS from fungi strain PH1008 isolated from mangrove on immune function in mice to evaluate its value in terms of immune function. Effects of EPS on the proliferation of murine splenic cell and its cytolytic activity were detected by MTT assay; Every mouse were administrated with different concentrations EPS through stomach for 28 days, calculate the indices of thymus, spleen and liver to investigate the effects of EPS on immune function in vitro. EPS stimulated the proliferation of spleen cell promoted its cytolytic activity in vitro, increased body index in mice and enhance the innate immune function. EPS from fungi strain PH1008 isolated from mangrove enhanced immune function in mice.
出处
《食品研究与开发》
CAS
北大核心
2015年第12期16-18,共3页
Food Research and Development
基金
国家自然科学基金资助(31260225)
海南省自然科学基金项目(309032)
