调节性B细胞在慢加急性肝衰竭小鼠中的免疫调节作用

Immunoregulatory role of IL-10-producing B cells in acute-on-chronic liver failure mice

目的研究调节性B细胞(Breg)对慢加急性肝衰竭(ACLF)小鼠的免疫调节作用。方法 CCl4诱导造ACLF模型,分离脾脏、肝脏、外周血淋巴细胞,流式细胞仪检测CD19+IL-10+B细胞比例,ELISA法检测IL-10表达水平。结果 ACLF组小鼠的24 h存活率低于对照组(20%vs 100%,P<0.001),ACLF小鼠肝脏、脾脏、外周血CD19+IL-10+B细胞比例高于对照组,分别为(2.97±0.33)%vs(0.75±0.11)%,(4.13±0.67)%vs(0.79±0.11)%,(5.87±1.00)%vs(0.68±0.09)%,差异均有统计学意义(均P<0.001)。ACLF小鼠肝脏、脾脏、外周血IL-10的分泌水平高于对照组,分别为(16.76±1.73)pg/ml vs(6.29±0.70)pg/ml,(21.21±1.62)pg/ml vs(8.23±1.52)pg/ml,(31.32±2.95)pg/ml vs(7.49±1.22)pg/ml,差异均有统计学意义(均P<0.001)。结论调节性B细胞可能在CCl4诱导的慢加急性肝衰竭小鼠疾病进展中起重要作用。

Objective To observe the immunoregulatory role of regulatory B cells （Breg） in Acute-on-Chronic Liver Failure （ACLF） mice. Methods Hepatic lymphocytes, splenic lymphocytes and peripheral blood lymphocytes were obtained from a total of thirty CCh-induced ACLF mice, and the percent of CD19^＋IL-10^＋ B cells were determined by flow cytometry （FCM）. IL-10 concentration in the lymphocytes culture supernatant was measured by ELISA. Results Compared to control group, the 24 hours overall survival rate of ACLF group was improved（20% vs 100%, P〈 .001 ）. And it was accompanied by a significantly increased number of IL-10-producing CD19^＋ B cells in liver, spleen and peripheral blood of ACLF mice （2.97±0.33）% vs （0.75±0.11 ）%, （4.13±0.67）% vs （0.79±0.11）%, （5.87±1.00）% vs （0.68±0.09）%,P〈0.001, respectively. The concentrations of IL-10 in the supernatant of cultured lymphocytes, from liver, spleen and peripheral blood, separately, were also elevated in ACLF mice（16.76±1.73）pg/m vs （6.29±0.70）pg/ml, （21.21±1.62）pg/m vs （8.23±1.52）pg/ml, （31.32±2.95）pg/m vs （7.49±1.22）pg,/ml, P〈0.001, respectively. Conehmion Our data indicated that increase in Breg cells and IL-10 concentration might play an critical role in the immunopathogenesis of ACLF.