柳栎浸膏提取物对抑制大鼠肾草酸钙结石形成的作用研究

Excluding stone efficiency of quercus salicina extract capsules on renal calcium oxalate mouse without renal function damage

目的探讨柳栎浸膏提取物对大鼠肾草酸钙结石形成的影响,并初步探讨其作用机制。方法采用乙二醇诱导大鼠肾草酸钙结石模型的建立,将60只Wistar大鼠随机分为对照组、模型组、柳栎浸膏低剂量组和柳栎浸膏高剂量组,每组15只,给药4周后,检测大鼠尿量、饮食量、体重,尿Ca2+、Mg2+、尿草酸和枸橼酸盐浓度,血肌酐(Cr)、尿素氮(BUN)和肾草酸浓度,肾脏中总超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果与模型组比较,柳栎浸膏低剂量组能降低大鼠的饮水量和尿量,降低尿钙[(1.89±0.43)mmol/L vs(3.43±0.20)mmol/L]和尿草酸浓度[(2.02±0.02)mmol/L vs(3.83±1.04)mmol/L],提高枸橼酸盐[(9.03±1.02)mmol/L vs(8.24±0.39)mmol/L]和Mg2+浓度[(16.30±0.23)mmol/L vs(6.13±1.44)mmol/L],降低Cr、BUN和草酸含量,提高SOD[(230.19±20.69)U/mg vs(160.83±9.05)U/mg]和GSH-Px活性[(45.53±5.45)U/mg vs(37.13±3.44)U/mg(均P<0.05);与柳栎浸膏低剂量组比较,高剂量组上述结果改变更显著。结论柳栎浸膏能够增加尿中结石抑制物,减少结石形成促进因素,从而抑制草酸钙结石的形成。

Objective To explore the excluding stone efficiency of quercussalicina extract capsules on renal calcium oxalate mouse without renal function damage. Methods Glycol was used to establish the model mouse with rental calcium oxalate. 60 Wistar mice were randomly divided into four groups including control group, model group, low-quercussalicina extract capsules group and high-quercussalicina extract capsules group. The urine volume, diet volume, Ca^2＋, Mg^2＋, oxalate, citrate concentration in urine, Cr, BUN contention in serum and SOD, GSH-Px activity were detected after feeding four weeks. Results Low-quercussalicina extract capsules could discrease the volume of urine and diet, decrease the concentration of Ca^2＋（ 1.89 ±0.43 ） mmol/L and oxalate （2.02±0.02） mmol/L in urine, increase the concentration of citrate （9.03 ±1.02） mmol/L and Mg^2＋（16.3±0.23） mmol/L, decrease the Cr, BUN and oxalate concentration and increase the activity of SOD （230.19±20.69）U/mg and GSH-Px （45.53 ±5A5）U/mg. In contrast, the results of high dose group changed more significantly. Conclusion Quercussalicina extract capsules can increase the stone-preventing effect and reduce the stone formation.