PCR-SSP法检测HPA-15基因频率的可靠性分析

Analysis on reliability of PCR-SSP method in detecting gene frequencies of HPA-15

目的探讨聚合酶链反应-序列特异性引物(PCR-SSP)法检测人类血小板抗原(HPA)-15基因的可靠性。方法设计序列特异性引物,采用PCR-SSP法对惠州地区100例无偿血小板献血者进行HPA-15基因分型,同时采用DNA测序法检测HPA-15基因频率,与PCR-SSP方法检测结果进行比对。结果 PCR-SSP法检测HPA-15a和HPA-15b的基因频率分别为0.535 0和0.465 0,DNA测序法检测HPA-15a和HPA-15b的基因频率分别为0.530 0和0.470 0,两种方法检测HPA-15基因频率差异无统计学意义(P>0.05)。结论 PCR-SSP方法可作为一种快速、便捷、可靠的筛查HPA-15基因频率技术,可为临床病人提供HPA-15相合的血小板。

Objective To investigate the reliability of polymerase chain reaction-sequence specific primers （PCR-SSP） method in detecting gene frequencies of human platelet antigen （HPA）-IS. Methods Sequence specific primers were designed for HPA-15 and PCR-SSP was employed to detect genotype of HPA-15. A total of 100 voluntary platelet donors were involved in the study. DNA sequencing was utilized to verify the genotype of HPA-15 by PCR-SSP simultaneously. Results Gene frequencies of HPA-15a and HPA-15b were found to be 0.535 0 and 0.465 0, respectively by PCR-SSP, and 0.530 0 and 0.470 0, respectively by DNA sequencing. The results difference of the two methods was statistically non-significant（P〉0.05）. Conclusion PCR-SSP method is proved to be a fast, convenient and reliable technique to detect the frequencies of HPA-15 and provide HPA-15 compatible donor platelets for patients.