不同振荡方式对FAME全自动酶免分析系统检测结果影响的分析

Effect of different modes of oscillation on ELISA results using the microlab FAME system

目的探讨进行ELISA实验时,不同振荡混匀方式对FAME全自动酶免系统检测结果的影响。方法选择HBs Ag试剂盒（A/B/C）、HCVAb试剂盒（a/b/c）在同一台全自动酶免系统进行匹配实验,根据振荡混匀参数不同,设置低速振荡（B/b）组和高速振荡（C/c）组,每组再根据振荡混匀时间（0、10、20、30 s）不同分为4个小组（B0~B3/b0~b3、C0~C3/c0~c3）,实验另设手工充分混匀对照（A/a）组,每组采用低值质控血清作为标本进行实验。计算各组（x±s）及变异系数（CV）,对最终的检测结果进行分析。结果 HBs Ag试剂B、C组结果与相应充分混匀A组比较,B0（0 s）、B1（10 s）、C0（0 s）、C1（10 s）组的结果差异有统计学意义（P〈0.05）;HCVAb试剂b、c组结果与相应充分混匀a组比较,b0（0 s）、c0（0 s）组的结果差异有统计学意义（P〈0.05）;振荡时间相同而振荡频率不同的B/b组和C/c组之间比较差异无统计学意义（P〉0.05）。结论振荡方式是全自动酶免系统运行的重要环节之一,其是否合理选择会直接影响ELISA实验结果的准确性。

Objective To discuss whether different modes of oscillation can affect the results of FAME automatic ELISA test. Methods According to different parameters of oscillation, selected HBsAg kit （A/B/C）, HCVAb kit （a/b/c） in the same automatic ELISA system; for matching experiments, low-speed group （B/b） and high-speed group （C/c） were selected. Each group was further divided into four different groups （B0-B3/b0-b3, C0-C3/c0-c3） by different blending time （0 s, 10 s, 20 s, 30 s）. In the experiment, manual group was used as control group （A/a）, and low value control serum as samples. The final test results of each group were calculated and analyzed by （x±s） and coefficient of variation （CV）. Results B and C groups in HBsAg reagents compared with the corresponding group A, there were statistically significant difference between group B0 （0 s）, B 1 （ 10 s）, CO （0 s）, C 1 （ 10 s） and control （P〈0.05） ; that was the same between group a with group b0 （0 s ）, c0（0 s） and control（P〈0.05） in HCVAb reagents. There were also no statistically significant difference between groups B/b and C/c （P〉0.05）. Conclusion The oscillation is one of the important aspects in automatic enzyme system operation, and the reasonable choice of oscillation modes directly affect the accuracy of ELISA experimental results.