深圳市生肉食品6种食源性致病菌污染状况调查

Surveillance on six foodborne pathogens in raw meat in Shenzhen

目的了解深圳市生肉食品受致病菌污染的状况,为食源性疾病的预防和食品安全预警提供科学依据。方法 2012-2013年对罗湖、福田、南山及宝安等8区有代表性的商场超市和农贸市场生肉食品随机采集576份样品,依据国标方法及全自动微生物分析仪,对上述样品进行致病菌检测。结果 576份样品共检出致病菌198株,检出率为34.38%,其中沙门菌105株、金黄色葡萄球菌60株、单增李斯特菌30株、空肠弯曲菌3株,未检出志贺菌和O157∶H7。312份禽肉类中检出致病菌70株,检出率为22.44%,以金黄色葡萄球菌污染为主;264份畜肉类中检出致病菌128株,检出率为48.48%,以沙门菌污染为主。畜肉类致病菌阳性检出率明显高于禽肉类,差异有统计学意义(χ2=43.01,P<0.01)。结论深圳市生肉食品受致病菌污染严重,以沙门菌、金黄色葡萄球菌和单核李斯特菌污染为主。商场超市和农贸市场生肉食品存在严重的食品安全问题,应加大对生肉食品监管力度,保证食品质量,确保消费者健康。

Objective To investigate and identify the contamination of main foodborne pathogens in Shenzhen, and provide a scientific basis for the prevention of foodborne disease and food safety warning. Methods A collection of 576 raw meat samples was obtained from supermarkets and farmers＇ market in Shenzhen using GB method and analyzed by automated microbiology analyzer. Results Among 576 samples, 198 （34.38%） pathogens strains were detected. In which, 105 isolates were Salmonella spp. 60 isolates were Staphylococcus aureus; 30 isolates were Listeria monocytogenes; 3 isolates were Campylobacterjejuni. Shigella and Escherichia coli O157 ： H7 were not detected. In 312 poultry samples, 70 （22.44%） pathogens strains were detected. Among 264 livestock meat samples, 128 （48.48%） pathogens strains were detected. Conclusion The status of contamination of raw meat by bacterial pathogens was severe in Shenzhen, in which were mainly contaminated by Salmonella, Staphylococcus aureus and Listeria monocytogenes. Therefore, preventive measures should be strengthened to ensure the food quality and health of consumers.