HPLC同时测定芪骨胶囊中5种成分的含量

Content Determination of Five Components in Qigu Capsules by HPLC

目的：建立芪骨胶囊的多成分指标定量方法。方法：采用高效液相色谱法同时测定芪骨胶囊中松果菊苷、朝藿定B,朝藿定C,淫羊藿苷和柚皮苷的含量,Platisil-C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-乙腈-水为流动相进行梯度洗脱,流速1 mL·min^-1,检测波长270 nm,柱温30℃。结果：松果菊苷,柚皮苷,朝藿定B,朝藿定C,淫羊藿苷分别在91～3 094 ng（r=0.999 8）,19～646 ng（r=0.999 7）,11～374 ng（r=0.999 5）,25～850 ng（r=0.999 5）,29～986 ng（r=0.999 6）与峰面积呈良好的线性关系;平均回收率分别为98.9%（RSD 1.2%）,99.1%（RSD 1.3%）,99.1%（RSD 1.5%）,99.4%（RSD 1.5%）,99.3%（RSD 1.5%）。结论：所建立的方法简便、准确、重复性好,可用于芪骨胶囊的质量控制。

Objective： To establish the multi-index quantitative method for Qigu capsules. Method：The contents of echinacoside,naringin,epimedin B,epimedin C and icariin were determined simultaneously by HPLC. Platisil-C18column（ 4. 6 mm × 250 mm,5 μm） was used and eluted with the mobile phase of acetonitrilemethanol-water in a gradient mode at the flow rate of 1 mL·min^-1. The detection wavelength was 270 nm,and the column temperature was 30 ℃. Result： Echinacoside,naringin,epimedin B,epimedin C,icariin showed a good linear relationship in the ranges of 91-3 094 ng（ r = 0. 999 8）,19-646 ng（ r = 0. 999 7）,11-374 ng（ r = 0. 999 5）,25-850 ng（ r = 0. 999 5）,29-986 ng（ r = 0. 999 6）,respectively. The average recoveries were 98. 9%（ RSD1. 2%）,99. 1%（ RSD 1. 3%）,99. 1%（ RSD 1. 5%）,99. 4%（ RSD 1. 5%）,99. 3%（ RSD 1. 5%）,respectively. Conclusion： This method is simple,accurate and highly reproducible,and so can be used to control the quality of Qigu capsules.